{"product_id":"plus-minus-12-13-dihome-elisa-kit-bhe19600077","title":"(±)12(13)-DiHOME ELISA Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eELISA Kit\u003c\/span\u003e\u003cp\u003e(±)12(13)-DiHOME is the vicinal diol metabolite of (±)12(13)-EpOME (i.e., isoleukotoxin), an epoxide derived from linoleic acid via cytochrome P450 epoxygenase activity. It has been shown to suppress neutrophil respiratory burst activity and exhibit selective cytotoxicity in certain cell lines expressing sEH. Thus, (±)12(13)-DiHOME bridges lipid metabolism and immune modulation, acting both as a downstream stress-responsive signal and as a paracrine regulator in inflammation and energy homeostasis.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eIntended Use The Tribioscience’s (±)12(13)-DiHOME ELISA Kit utilizes competitive ELISA technologies for the quantitative analysis of (±)12(13)-DiHOME in biological samples. It provides a vital tool to accurately measure (±)12(13)-DiHOME with applications in inflammation, metabolism, and respiratory research. The assay has a range of 0.14-100 ng\/ml.\u003c\/p\u003e\n\u003cp\u003eTypical Data This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003eSimple Procedures (Fig. 1) Simple Standard Preparation\u003c\/p\u003e\n\u003cp\u003e(±)12(13)-DiHOME ELISA Kit is a Competitive ELISA for the quantitative determination of (±)12(13)-DiHOME. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eThe kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003e(±)12(13)-DiHOME\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eCompetitive\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTest Principle\u003c\/th\u003e\n\u003ctd\u003eCompetitive ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.14–100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore the unopened kit at 2-8°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✓ Quality tested for reproducibility and lot-to-lot consistency\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices and consult the SDS before handling.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 96-well plate","offer_id":53547749867885,"sku":"TBS32129","price":460.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TBS32129-600x600.jpg?v=1783980660","url":"https:\/\/www.ebiohippo.com\/products\/plus-minus-12-13-dihome-elisa-kit-bhe19600077","provider":"BioHippo","version":"1.0","type":"link"}