{"product_id":"pmvk-antibody-phosphomevalonate-kinase-bha17135691","title":"PMVK Antibody \/ Phosphomevalonate kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePMVK Antibody \/ Phosphomevalonate kinase is a anti-PMVK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PMVK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePMVK antibody detects Phosphomevalonate kinase, a key enzyme in the mevalonate pathway responsible for cholesterol and isoprenoid biosynthesis. Encoded by the PMVK gene on chromosome 1q21.3, this cytosolic enzyme catalyzes the ATP-dependent phosphorylation of mevalonate 5-phosphate to mevalonate 5-diphosphate, an essential step linking early mevalonate metabolism to downstream sterol and non-sterol isoprenoid synthesis. The mevalonate pathway provides precursors for cholesterol, dolichols, ubiquinone, and prenylated proteins that regulate membrane integrity and signal transduction.\u003cbr\u003e\u003cbr\u003ePMVK operates downstream of HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis. Its activity is tightly controlled by cellular sterol levels and feedback mechanisms that balance lipid production with energy demands. Beyond lipid metabolism, PMVK influences cell proliferation, differentiation, and apoptosis by modulating isoprenoid-dependent signaling pathways. It localizes to both the cytosol and peroxisomes, reflecting its versatile role in cellular metabolism.\u003cbr\u003e\u003cbr\u003eThe PMVK antibody is widely used in metabolism, lipid biology, and pharmacology research to study cholesterol regulation and mevalonate pathway dynamics. Western blot analysis detects a 21 kilodalton band corresponding to PMVK, while immunofluorescence shows cytoplasmic and peroxisomal staining. This antibody enables quantification of PMVK expression in response to statin treatment or metabolic stress, supporting studies of lipid homeostasis and biosynthetic control.\u003cbr\u003e\u003cbr\u003eAltered PMVK expression has been linked to hypercholesterolemia, metabolic disorders, and cancer cell survival, where mevalonate flux influences oncogenic signaling and membrane biosynthesis. The PMVK antibody is a valuable reagent for characterizing cholesterol metabolism, isoprenoid synthesis, and metabolic adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PMVK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PMVK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PMVK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PMVK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PMVK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299604845,"sku":"FY12789","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_34c47d89-05e5-4b3f-968a-c77422b1438f.jpg?v=1782237047","url":"https:\/\/www.ebiohippo.com\/products\/pmvk-antibody-phosphomevalonate-kinase-bha17135691","provider":"BioHippo","version":"1.0","type":"link"}