{"product_id":"pold1-antibody-dna-polymerase-delta-catalytic-subunit-bha17135812","title":"POLD1 Antibody \/ DNA polymerase delta catalytic subunit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLD1 Antibody \/ DNA polymerase delta catalytic subunit is a anti-POLD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLD1 antibody detects DNA polymerase delta catalytic subunit, the core enzyme responsible for high-fidelity DNA synthesis and repair during replication. Encoded by the POLD1 gene on chromosome 19q13.33, this enzyme serves as the catalytic and proofreading component of DNA polymerase delta, a multi-subunit complex essential for lagging-strand synthesis, mismatch repair, and genomic stability. POLD1 possesses both 5'-3' polymerase and 3'-5' exonuclease proofreading activities, ensuring accurate DNA replication and error correction.\u003cbr\u003e\u003cbr\u003eStructurally, POLD1 is a large enzyme of approximately 125 kilodaltons containing conserved polymerase motifs and an N-terminal exonuclease domain. It forms a heterotetrameric complex with POLD2, POLD3, and POLD4 subunits, supported by interactions with PCNA (proliferating cell nuclear antigen) and replication factor C. This complex coordinates DNA elongation, repair synthesis, and recombination processes, playing a vital role in cell cycle progression and genome maintenance.\u003cbr\u003e\u003cbr\u003eThe POLD1 antibody is widely used in molecular biology, cancer research, and DNA repair studies to investigate replication fidelity, mutagenesis, and polymerase function. Western blot analysis detects a 125 kilodalton band corresponding to the catalytic subunit, while immunofluorescence shows nuclear localization during S phase and DNA damage repair. This antibody provides a robust tool for assessing DNA replication machinery and the maintenance of genomic integrity.\u003cbr\u003e\u003cbr\u003eMutations in POLD1 cause Polymerase Proofreading-Associated Polyposis and predispose to colorectal and endometrial cancers due to defective proofreading activity and increased mutational load. Additionally, POLD1 variants are linked to syndromes involving premature aging and mitochondrial dysfunction. Its role extends beyond replication to DNA damage tolerance and translesion synthesis. The POLD1 antibody supports exploration of polymerase fidelity mechanisms, DNA repair pathways, and tumor suppressor networks involving replication stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306420589,"sku":"FY12910","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ad7c34aa-97b4-42d7-b9d8-84d09852ee75.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/pold1-antibody-dna-polymerase-delta-catalytic-subunit-bha17135812","provider":"BioHippo","version":"1.0","type":"link"}