{"product_id":"pold4-antibody-dna-polymerase-delta-subunit-4-bha17135219","title":"POLD4 Antibody \/ DNA polymerase delta subunit 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLD4 Antibody \/ DNA polymerase delta subunit 4 is a anti-POLD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLD4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLD4 antibody detects DNA polymerase delta subunit 4, encoded by the POLD4 gene on chromosome 11q13.4. POLD4 antibody is widely applied in DNA replication, repair, and cell cycle research. POLD4 is a small accessory subunit of DNA polymerase delta, a multi-subunit complex essential for lagging strand DNA synthesis, Okazaki fragment maturation, and DNA repair. POLD4 stabilizes the polymerase complex and enhances its processivity in conjunction with PCNA and replication factor C.\u003cbr\u003e\u003cbr\u003eStructurally, POLD4 is a ~12 kDa protein with no catalytic activity but essential stabilizing functions. It interacts with the catalytic subunit POLD1 and accessory subunits POLD2 and POLD3, forming the tetrameric polymerase delta complex. By supporting proper assembly, POLD4 ensures high-fidelity DNA replication and repair.\u003cbr\u003e\u003cbr\u003eFunctionally, POLD4 contributes to DNA replication, mismatch repair, and recombination. It coordinates lagging strand synthesis, working with FEN1 and DNA ligase I to process Okazaki fragments. Researchers use POLD4 antibody to study DNA replication, checkpoint control, and repair pathways.\u003cbr\u003e\u003cbr\u003eClinically, defects in DNA polymerase delta subunits including POLD4 have been linked to cancer, immunodeficiency, and genomic instability syndromes. Reduced POLD4 expression impairs DNA replication fidelity, contributing to mutagenesis. Because DNA replication is a key therapeutic target, POLD4 is being investigated in oncology. \u003cbr\u003e\u003cbr\u003eExperimentally, POLD4 antibody is applied in western blotting to detect the ~12 kDa protein, in immunoprecipitation to isolate the polymerase delta complex, and in immunofluorescence to study nuclear localization. Chromatin immunoprecipitation with POLD4 antibody supports research on replication fork progression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLD4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLD4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLD4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLD4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLD4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285252461,"sku":"FY12316","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_283cee3a-1084-4a91-a55d-50058d948d52.jpg?v=1782236986","url":"https:\/\/www.ebiohippo.com\/products\/pold4-antibody-dna-polymerase-delta-subunit-4-bha17135219","provider":"BioHippo","version":"1.0","type":"link"}