{"product_id":"poldip2-antibody-polymerase-delta-interacting-protein-2-bha17136068","title":"POLDIP2 Antibody \/ Polymerase delta-interacting protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLDIP2 Antibody \/ Polymerase delta-interacting protein 2 is a anti-POLDIP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLDIP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLDIP2 antibody detects Polymerase delta-interacting protein 2, a multifunctional protein that regulates DNA replication, repair, and mitochondrial function. The UniProt recommended name is Polymerase delta-interacting protein 2 (POLDIP2). This adaptor protein enhances the activity of DNA polymerase delta and coordinates cellular redox balance, cytoskeletal organization, and oxidative stress responses.\u003cbr\u003e\u003cbr\u003eFunctionally, POLDIP2 antibody identifies a 368-amino-acid protein localized to the nucleus, cytoplasm, and mitochondria. POLDIP2 binds to DNA polymerase delta subunit p50, stimulating processive DNA synthesis. It also interacts with NADPH oxidase 4 (NOX4), promoting the production of reactive oxygen species for signaling and host defense. In mitochondria, POLDIP2 influences respiratory complex stability and oxidative phosphorylation efficiency.\u003cbr\u003e\u003cbr\u003eThe POLDIP2 gene is located on chromosome 17q11.2 and is broadly expressed in metabolically active tissues, including heart, liver, and brain. Its expression is induced by stress signals and growth factors, integrating DNA repair, redox signaling, and metabolic regulation.\u003cbr\u003e\u003cbr\u003ePathologically, altered POLDIP2 expression has been linked to vascular remodeling, fibrosis, cancer, and neurodegenerative disorders. Overexpression promotes proliferation and migration in vascular smooth muscle cells, while deficiency impairs mitochondrial respiration and DNA replication fidelity. Research using POLDIP2 antibody supports studies in DNA metabolism, oxidative stress, and mitochondrial biology.\u003cbr\u003e\u003cbr\u003ePOLDIP2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect replication-associated and redox-regulating proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Polymerase delta-interacting protein 2 contains an N-terminal mitochondrial targeting sequence and a YccV-like domain involved in protein-protein interactions. This antibody facilitates investigation of POLDIP2's role in coordinating nuclear and mitochondrial stress responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLDIP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLDIP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLDIP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLDIP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLDIP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314612589,"sku":"FY13166","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a7a15bf4-2465-49d9-b034-44f72ca15a61.jpg?v=1782237103","url":"https:\/\/www.ebiohippo.com\/products\/poldip2-antibody-polymerase-delta-interacting-protein-2-bha17136068","provider":"BioHippo","version":"1.0","type":"link"}