{"product_id":"polg2-antibody-dna-polymerase-subunit-gamma-2-bha17135542","title":"POLG2 Antibody \/ DNA polymerase subunit gamma-2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLG2 Antibody \/ DNA polymerase subunit gamma-2 is a anti-POLG2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLG2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLG2 antibody detects DNA polymerase subunit gamma-2, a key accessory subunit of the mitochondrial DNA polymerase complex responsible for mitochondrial genome replication and repair. POLG2 enhances the processivity and stability of the catalytic subunit POLG, ensuring faithful replication of mitochondrial DNA (mtDNA). The POLG2 antibody is widely used in mitochondrial biology, genetics, and aging research to study DNA maintenance, oxidative stress, and mitochondrial dysfunction.\u003cbr\u003e\u003cbr\u003ePOLG2 is encoded by the POLG2 gene located on human chromosome 17q23.3. The protein is approximately 485 amino acids in length and localizes to the mitochondrial matrix. It forms a homodimer that binds to POLG, increasing its affinity for DNA and reducing dissociation during replication. Through this interaction, POLG2 coordinates mtDNA replication and repair under both physiological and stress conditions.\u003cbr\u003e\u003cbr\u003eThe POLG2 antibody detects a 55 kilodalton band by western blot and shows mitochondrial punctate staining under immunofluorescence microscopy. Loss-of-function mutations in POLG2 result in reduced mtDNA copy number, leading to multiple mitochondrial DNA deletions, oxidative stress, and mitochondrial depletion syndromes. Clinical manifestations include progressive external ophthalmoplegia, ataxia, and myopathy.\u003cbr\u003e\u003cbr\u003ePOLG2 also contributes to mitochondrial biogenesis and interacts with nucleoid proteins such as TFAM and TWINKLE, supporting DNA packaging and maintenance. Dysregulation of POLG2 expression has been observed in neurodegenerative diseases, aging tissues, and metabolic disorders, where impaired mtDNA replication leads to reduced energy output and increased reactive oxygen species production.\u003cbr\u003e\u003cbr\u003eBecause of its essential role in maintaining mitochondrial genome integrity, POLG2 serves as a critical factor in understanding mitochondrial pathophysiology and age-related dysfunction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLG2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLG2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLG2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLG2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLG2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294820717,"sku":"FY12639","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_05da1184-a3e4-4ca5-8413-6d5032a16a1b.jpg?v=1782237019","url":"https:\/\/www.ebiohippo.com\/products\/polg2-antibody-dna-polymerase-subunit-gamma-2-bha17135542","provider":"BioHippo","version":"1.0","type":"link"}