{"product_id":"polm-antibody-dna-polymerase-mu-bha17135986","title":"POLM Antibody \/ DNA polymerase mu","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLM Antibody \/ DNA polymerase mu is a anti-POLM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLM antibody detects DNA polymerase mu, a specialized DNA polymerase involved in non-homologous end joining (NHEJ) and DNA repair. The UniProt recommended name is DNA polymerase mu (POLM). This enzyme belongs to the X family of DNA polymerases, functioning at sites of double-strand breaks to fill in short gaps and process damaged DNA ends during repair.\u003cbr\u003e\u003cbr\u003eFunctionally, POLM antibody identifies a 494-amino-acid nuclear enzyme that exhibits template-dependent and template-independent polymerase activities. POLM incorporates nucleotides opposite damaged or missing bases, ensuring ligation competency of DNA ends. Unlike high-fidelity polymerases, POLM lacks strong proofreading activity, allowing it to tolerate mismatched or gapped templates-a property critical for repair flexibility in NHEJ.\u003cbr\u003e\u003cbr\u003eThe POLM gene is located on chromosome 7p13 and is expressed in immune and proliferative tissues such as thymus and bone marrow. It plays a key role in V(D)J recombination and class switch recombination in lymphocytes by repairing programmed double-strand breaks. Its activity complements DNA polymerase lambda (POLL) in filling in short DNA overhangs during repair.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of POLM contributes to genomic instability and may influence cancer development, immune function, and cellular aging. Overexpression can increase mutation rates, whereas deficiency impairs NHEJ efficiency. POLM's balance between flexibility and error rate is vital for maintaining genome integrity while allowing adaptability in immune diversification.\u003cbr\u003e\u003cbr\u003ePOLM antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA polymerase mu in nuclear extracts. \u003cbr\u003e\u003cbr\u003eStructurally, DNA polymerase mu contains an N-terminal BRCT domain mediating protein-protein interactions, followed by a catalytic polymerase domain with palm, fingers, and thumb subdomains typical of X-family enzymes. This antibody supports studies on POLM's mechanistic role in end joining, mutation repair, and adaptive immunity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312023917,"sku":"FY13084","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f2de3a74-9a96-467e-8655-2a4dae448e8a.jpg?v=1782237086","url":"https:\/\/www.ebiohippo.com\/products\/polm-antibody-dna-polymerase-mu-bha17135986","provider":"BioHippo","version":"1.0","type":"link"}