{"product_id":"polr1a-antibody-dna-directed-rna-polymerase-i-subunit-rpa1-bha17135757","title":"POLR1A Antibody \/ DNA-directed RNA polymerase I subunit RPA1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLR1A Antibody \/ DNA-directed RNA polymerase I subunit RPA1 is a anti-POLR1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLR1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLR1A antibody detects DNA-directed RNA polymerase I subunit RPA1, the largest catalytic subunit of RNA polymerase I, which is responsible for ribosomal RNA (rRNA) synthesis in the nucleolus. Encoded by the POLR1A gene on chromosome 2p11.2, this essential enzyme subunit forms part of the multi-protein RNA polymerase I complex that catalyzes transcription of 45S pre-rRNA, a key step in ribosome biogenesis. POLR1A serves as the catalytic core that binds DNA and nucleoside triphosphates, driving polymerization during transcription initiation and elongation.\u003cbr\u003e\u003cbr\u003eStructurally, POLR1A contains a DNA-binding cleft, catalytic residues within the active center, and multiple contact surfaces for interacting with transcription factors and other RNA polymerase I subunits. It forms the backbone of the enzyme and interacts with RPA2 and RPA3 to establish the core complex. POLR1A also associates with transcription initiation factors such as SL1 and UBF, which guide polymerase recruitment to rDNA promoters and regulate transcriptional output in response to growth signals.\u003cbr\u003e\u003cbr\u003eThe POLR1A antibody is widely used in transcription, cell growth, and cancer biology research to study ribosomal biogenesis and nucleolar function. Western blot analysis detects a 190 kilodalton band corresponding to POLR1A, while immunofluorescence reveals strong nucleolar staining consistent with its role in rRNA synthesis. This antibody supports studies of transcriptional regulation, nucleolar stress responses, and metabolic control of protein synthesis.\u003cbr\u003e\u003cbr\u003ePOLR1A activity is tightly linked to cellular growth and proliferation. Under conditions of nutrient abundance, mTOR signaling promotes RNA polymerase I activation and rRNA transcription; conversely, DNA damage or stress inhibits POLR1A function to conserve resources. Mutations in POLR1A cause ribosomopathies such as acrofacial dysostosis, characterized by craniofacial malformations due to impaired ribosome production. Overactivation of RNA polymerase I, on the other hand, contributes to oncogenic transformation by driving excessive ribosome biogenesis and protein synthesis. The POLR1A antibody allows precise monitoring of polymerase expression and localization under these physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLR1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLR1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLR1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLR1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLR1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304552813,"sku":"FY12855","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2ec005d5-1640-490f-aac8-84f0d2f9180f.jpg?v=1782237054","url":"https:\/\/www.ebiohippo.com\/products\/polr1a-antibody-dna-directed-rna-polymerase-i-subunit-rpa1-bha17135757","provider":"BioHippo","version":"1.0","type":"link"}