{"product_id":"polr1e-antibody-dna-directed-rna-polymerase-i-subunit-e-paf53-bha17135646","title":"POLR1E Antibody \/ DNA-directed RNA polymerase I subunit E \/ PAF53","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLR1E Antibody \/ DNA-directed RNA polymerase I subunit E \/ PAF53 is a anti-POLR1E Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLR1E\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLR1E antibody detects DNA-directed RNA polymerase I subunit E (also known as PAF53), a core component of the RNA polymerase I complex responsible for synthesizing ribosomal RNA (rRNA) precursors. Encoded by the POLR1E gene on chromosome 9q34.11, this protein is essential for rRNA transcription and ribosome biogenesis. POLR1E interacts with other polymerase I subunits, transcription initiation factors, and nucleolar scaffolding proteins to regulate transcription of the 47S rRNA gene cluster. By supporting nucleolar organization and transcriptional efficiency, it plays a fundamental role in cell growth and proliferation.\u003cbr\u003e\u003cbr\u003ePOLR1E localizes to the nucleolus and associates with the transcription initiation factor SL1 and upstream binding factor (UBF) to form a preinitiation complex at rDNA promoters. Its C-terminal domain mediates binding to RNA polymerase I subunit RPA1 and other core enzyme components, facilitating enzyme assembly and promoter clearance. Loss of POLR1E disrupts nucleolar architecture and reduces rRNA synthesis, leading to impaired ribosome production and cell-cycle arrest. This makes it a critical determinant of nucleolar integrity and growth control.\u003cbr\u003e\u003cbr\u003eThe POLR1E antibody is widely used in molecular biology, cancer research, and nucleolar studies to assess RNA polymerase I activity and rRNA transcription regulation. Western blot analysis identifies a 53 kilodalton band corresponding to POLR1E, while immunofluorescence reveals punctate nucleolar staining. Because RNA polymerase I transcription drives ribosome production, alterations in POLR1E expression are linked to tumor growth and metabolic adaptation. Increased expression has been observed in rapidly dividing cells, whereas downregulation occurs under cellular stress or growth inhibition.\u003cbr\u003e\u003cbr\u003eIn addition to its role in transcription, POLR1E contributes to the nucleolar stress response by coordinating signaling between rRNA synthesis and p53 activation. Defects in polymerase I assembly or activity trigger nucleolar stress pathways that stabilize p53, resulting in growth arrest. The POLR1E antibody enables exploration of these mechanisms and supports investigations into ribosomopathies, tumorigenesis, and cellular aging.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLR1E - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLR1E - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLR1E - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLR1E - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLR1E+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298720109,"sku":"FY12743","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b433df8a-50cb-4206-bedd-bf7d22388b67.jpg?v=1782237035","url":"https:\/\/www.ebiohippo.com\/products\/polr1e-antibody-dna-directed-rna-polymerase-i-subunit-e-paf53-bha17135646","provider":"BioHippo","version":"1.0","type":"link"}