{"product_id":"polr3b-antibody-bha10531604","title":"POLR3B Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-POLR3B antibody raised in Rabbit, tested in ELISA, IHC. Designed to detect POLR3B in Human for epigenetics and nuclear signaling research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human DNA-directed RNA polymerase III subunit RPC2 protein (831-939AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003ePOLR3B\u003c\/strong\u003e (also: C128, DNA directed RNA polymerase III 127.6 kDa polypeptide, DNA directed RNA polymerase III subunit B, DNA-directed RNA polymerase III 127.6 kDa polypeptide, DNA-directed RNA polymerase III subunit B, DNA-directed RNA polymerase III subunit RPC2, FLJ10388, polr3b, Polymerase (RNA) III (DNA directed) polypeptide B, RNA polymerase III subunit C2, RNA polymerase III subunit RPC2, RPC2, RPC2_HUMAN) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current POLR3B annotation relevant to epigenetics and nuclear signaling.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Tissue expression in FFPE\/frozen sections; optimize antigen retrieval.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241108824429,"sku":"CSB-PA885742LA01HU-50UG","price":166.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241200050541,"sku":"CSB-PA885742LA01HU-100UG","price":299.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA885742LA01HU-IHC-1.jpg?v=1776692860","url":"https:\/\/www.ebiohippo.com\/products\/polr3b-antibody-bha10531604","provider":"BioHippo","version":"1.0","type":"link"}