{"product_id":"polr3d-antibody-dna-directed-rna-polymerase-iii-subunit-rpc4-bha17136360","title":"POLR3D Antibody \/ DNA-directed RNA polymerase III subunit RPC4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOLR3D Antibody \/ DNA-directed RNA polymerase III subunit RPC4 is a anti-POLR3D Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POLR3D\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOLR3D antibody is a key reagent for studying transcriptional regulation and RNA metabolism. The encoded protein, DNA-directed RNA polymerase III subunit RPC4, is an essential component of RNA polymerase III, the enzyme complex responsible for synthesizing small structural RNAs including tRNAs, 5S rRNA, and other short non-coding RNAs. These transcripts are fundamental to ribosome biogenesis, protein synthesis, and multiple regulatory pathways. By supporting the assembly and activity of RNA polymerase III, POLR3D ensures proper cellular growth, proliferation, and maintenance of homeostasis.\u003cbr\u003e\u003cbr\u003eDNA-directed RNA polymerase III subunit RPC4 contributes to the conserved catalytic core of the enzyme, providing structural stability and facilitating transcriptional activity. Its interactions with other polymerase subunits form part of the framework that enables RNA chain initiation and elongation. Beyond its basal role, POLR3D helps modulate transcription in response to signaling pathways, linking environmental and metabolic cues to RNA production.\u003cbr\u003e\u003cbr\u003eMutations in DNA-directed RNA polymerase III subunit RPC4 have been associated with rare neurodevelopmental syndromes, underscoring its importance in human biology. Furthermore, RNA polymerase III and its subunits, including POLR3D, are closely tied to innate immune function. Viral infections often exploit RNA polymerase III activity, while host defenses detect RNA polymerase III products as part of antiviral recognition systems. This highlights the dual role of POLR3D in normal physiology and immune response.\u003cbr\u003e\u003cbr\u003eCancer research has also implicated RNA polymerase III dysregulation, where increased transcription of tRNAs and 5S rRNA drives uncontrolled protein synthesis and cellular proliferation. As part of this complex, DNA-directed RNA polymerase III subunit RPC4 may contribute to tumor development by supporting excessive biosynthetic activity. Ongoing studies are examining whether POLR3D and its associated pathways can serve as biomarkers or therapeutic targets.\u003cbr\u003e\u003cbr\u003eThe POLR3D antibody is applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to assess expression, localization, and changes in disease or experimental models. These tools are vital for researchers investigating transcriptional regulation, immune surveillance, and oncogenic pathways. For advanced molecular studies requiring consistent and accurate detection, the POLR3D antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POLR3D - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POLR3D - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POLR3D - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POLR3D - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POLR3D+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326441837,"sku":"RQ8942","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b4347d2e-98c0-482c-99cb-0e3d69570fc6.jpg?v=1772019449","url":"https:\/\/www.ebiohippo.com\/products\/polr3d-antibody-dna-directed-rna-polymerase-iii-subunit-rpc4-bha17136360","provider":"BioHippo","version":"1.0","type":"link"}