{"product_id":"poly-a-polymerase-yeast-bhz10900002","title":"Poly(A) Polymerase, Yeast","description":"\u003cp\u003e\u003cstrong\u003ePoly(A) Polymerase, Yeast\u003c\/strong\u003e catalyzes the template-independent addition of adenosine residues to the 3’ ends of RNA molecules using ATP as a substrate to create a poly(A) tail. When cordycepin-5'-triphosphate (3’-dATP) is substituted for ATP, the enzyme adds a single dA residue to the 3’-terminus of RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast is particularly effective for \u003cstrong\u003eoligonucleotide-labeling and poly(A) tailing of long RNA templates\u003c\/strong\u003e, making it a superior choice compared to E. coli-derived enzymes. This enzyme is ideal for applications that require \u003cstrong\u003eprecise polyadenylation,\u003c\/strong\u003e such as \u003cstrong\u003eRNA sequencing\u003c\/strong\u003e and \u003cstrong\u003ecDNA synthesis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin: 20px 0;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eProduct Component\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003ePoly(A) Polymerase, Yeast (1 U\/µl)\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5X Poly(A) Polymerase, Yeast Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e1.0 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e25 mM MnCl₂\u003c\/td\u003e\n\u003ctd\u003e500 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATP (10 mM)\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\u003ctable class=\"bhc-specs-table\" style=\"width:100%;border-collapse:collapse\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#1a5c58;color:#fff\"\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eEnzyme Type\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003ePoly(A) Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eEnzyme Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eStore all components at -20°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 U (100 ul)","offer_id":53294164672877,"sku":"E017","price":125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/poly-a--polymerase-yeast_v2.png?v=1778166207","url":"https:\/\/www.ebiohippo.com\/products\/poly-a-polymerase-yeast-bhz10900002","provider":"BioHippo","version":"1.0","type":"link"}