{"product_id":"polyjet-in-vitro-dna-transfection-reagent-bht2200062","title":"PolyJet™ In Vitro DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePolyJet™ DNA In Vitro Transfection Reagent rests on SignaGen's proprietary polymer synthesis technology and takes the form of a biodegradable, polymer-based DNA transfection reagent, giving effective and reproducible results in CHO, HeLa, NIH-3T3, COS-7, HEK293, and a wide span of mammalian cells that resist transfection. Even at very low concentration it halts DNA migration in electrophoresis, and the transfection complex is ready within 5 minutes at RT. What stands out is how the polymer breaks down, rapidly and completely, once the transfection complex has undergone endocytosis, leaving cytotoxicity much lower.\u003c\/p\u003e\n\u003cp\u003eA 1.0 ml quantity of PolyJet™ reagent stretches to ~667 transfections in 24-well plates or ~333 in 6-well plates, which SignaGen positions as a very affordable alternative to the leading products where commonly used as well as hard-to-transfect mammalian cells must be transfected.\u003c\/p\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_Degradation_jpg.webp?v=1790173423\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – polymer degradation after endocytosis illustration\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 1. A Cartoon Showing Biodegradation of PolyJet™ DNA Transfection Reagent After Endocytosis of Transfection Complex\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBiodegradable once endocytosed\u003c\/li\u003e\n\u003cli\u003eExceptionally high titers of virus production\u003c\/li\u003e\n\u003cli\u003eWorks equally well with very long DNAs (\u0026gt;89 kb)\u003c\/li\u003e\n\u003cli\u003eEqually suited to single DNA transfection and multi DNA co-transfection\u003c\/li\u003e\n\u003cli\u003eHigh-level recombinant protein production\u003c\/li\u003e\n\u003cli\u003eStraightforward, robust transfection procedure\u003c\/li\u003e\n\u003cli\u003eVery low cost\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet-CHO_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – HA-beta-tubulin expression in CHO cells vs lipofectamine Plus\" loading=\"lazy\"\u003e\u003cfigcaption\u003eTransfection efficiency comparison of PolyJet™ vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyJet™ (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). The above picture was provided by Dr. Shang Yin of University of Texas at Houston Medical School as courtesy\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJetvsL2K-HEK293_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in HEK293FT cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJetvsL2K-HepG2_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in HepG2 cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison showing transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_MDCK2_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in MDCK cells vs Fugene HD with DAPI staining\" loading=\"lazy\"\u003e\u003cfigcaption\u003eTransfection efficiency comparison of PolyJet™ vs. Fugene HD on MDCK cells. A plain GFP DNA was transduced into MDCK cells with PolyJet™ (left panel) and Fugene HD (right panel) reagents, respectively per the manufacturers’ protocols. GFP and DAPI staining were visualized under fluorescence microscopy 48 hours post-transfection. The above comparison data and pictures were completed and provided by Dr. Ge Zhou of NYU Medical Center as courtesy\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_MDCK_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in MDCK cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary “Shaved Cell Transfection” protocol, PolyJet™ (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel), around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_LNCAP_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in LNCap cells vs Fugene HD\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Fugene HD on LNCap cells. LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Fugene HD (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_hESCs_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in human embryonic stem cells (hESCs), DIC and fluorescence\" loading=\"lazy\"\u003e\u003cfigcaption\u003eAn image showing the exceptional transfection efficiency of PolyJet™ reagent on Human embryonic stem cells (hESCs). The hESCs grown in E8 medium on Geltrexvs (left panel, DIC imaging) was transfected with pEF1α-GFP. The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post-transfection. The above pictures were provided by Dr. Marina Pryzhkova of Johns Hopkins University as a courtesy\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet-2_jpg.webp?v=1790173499\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – GFP transfection in Neuro2A cells, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cfigcaption\u003eNeuro2A cells transfected with pEGFP-C1 plasmid using PolyJet™ In Vitro DNA Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet-T_jpg.webp?v=1790173498\" alt=\"PolyJet™ In Vitro DNA Transfection Reagent – cytotoxicity vs L2K™ in primary murine skin fibroblasts, DIC imaging\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of cytotoxicity of PolyJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents\/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium, followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch3\u003eTransfection Efficiency by Cell Line\u003c\/h3\u003e\n\u003ctable class=\"bhp-spec-table bhp-data-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eEfficiency (% GFP)\u003c\/th\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eEfficiency (% GFP)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMcArdle 7777\u003c\/td\u003e\n\u003ctd\u003e65-70%\u003c\/td\u003e\n\u003ctd\u003ehESCs\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHep3D\u003c\/td\u003e\n\u003ctd\u003e67-76%\u003c\/td\u003e\n\u003ctd\u003eSN56\u003c\/td\u003e\n\u003ctd\u003e81%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSHEP\u003c\/td\u003e\n\u003ctd\u003e68-71%\u003c\/td\u003e\n\u003ctd\u003eMC3T3-E1\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3T3-442A\u003c\/td\u003e\n\u003ctd\u003e35%\u003c\/td\u003e\n\u003ctd\u003ePrimary melanocyte\u003c\/td\u003e\n\u003ctd\u003e35%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCOS-7\u003c\/td\u003e\n\u003ctd\u003e85-90%\u003c\/td\u003e\n\u003ctd\u003emESCs\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCV-1\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003eL929\u003c\/td\u003e\n\u003ctd\u003e59%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD 407\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003eMCF-7\u003c\/td\u003e\n\u003ctd\u003e68%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDHD Pro.b\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003ctd\u003e68%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3LL\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003eNeuro2A\u003c\/td\u003e\n\u003ctd\u003e86%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eB16-F10\u003c\/td\u003e\n\u003ctd\u003e85%\u003c\/td\u003e\n\u003ctd\u003eNIH 3T3\u003c\/td\u003e\n\u003ctd\u003e76%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBAEC\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003ctd\u003ePC12\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBHK-21\u003c\/td\u003e\n\u003ctd\u003e80%\u003c\/td\u003e\n\u003ctd\u003eSH-SY5Y\u003c\/td\u003e\n\u003ctd\u003e25%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCa Ski\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003ctd\u003eSiHa\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCaCo2\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003eSKOV3\u003c\/td\u003e\n\u003ctd\u003e65%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003ctd\u003eHuh-7\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHCS-2\/8\u003c\/td\u003e\n\u003ctd\u003e61%\u003c\/td\u003e\n\u003ctd\u003eIGROV1\u003c\/td\u003e\n\u003ctd\u003e35%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEK-293\u003c\/td\u003e\n\u003ctd\u003e86%\u003c\/td\u003e\n\u003ctd\u003eDF-1, Chicken Embryonic Cell\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeLa\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003ctd\u003e6CSFMEo\u003c\/td\u003e\n\u003ctd\u003e71%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHLMEC\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003ctd\u003eWEHI 231\u003c\/td\u003e\n\u003ctd\u003e26%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH-MVEC\u003c\/td\u003e\n\u003ctd\u003e59%\u003c\/td\u003e\n\u003ctd\u003eA549\u003c\/td\u003e\n\u003ctd\u003e75%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuh-7D12\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003ctd\u003eLNCap\u003c\/td\u003e\n\u003ctd\u003e75\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATT20\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003ctd\u003ePrim. mouse keratinocyte\u003c\/td\u003e\n\u003ctd\u003e29%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSK-N-SH\u003c\/td\u003e\n\u003ctd\u003e29%\u003c\/td\u003e\n\u003ctd\u003ePrim. human skin fibroblast\u003c\/td\u003e\n\u003ctd\u003e50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003ctd\u003ePrim. human pre-adipocyte\u003c\/td\u003e\n\u003ctd\u003e32%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003ctd\u003ePrim. mouse embry. fibroblast\u003c\/td\u003e\n\u003ctd\u003e30%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900660589,"sku":"SL100688-0P1ML","price":23.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905674093,"sku":"SL100688-0P5ML","price":78.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905706861,"sku":"SL100688-1P0ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905739629,"sku":"SL100688-1P0MLX5","price":707.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PolyJet_Degradation_jpg_4e116e5a-bb06-4f67-99f3-95ddd02e56a7.webp?v=1790175949","url":"https:\/\/www.ebiohippo.com\/products\/polyjet-in-vitro-dna-transfection-reagent-bht2200062","provider":"BioHippo","version":"1.0","type":"link"}