{"product_id":"pomgnt2-antibody-protein-o-linked-mannose-n-acetylglucosaminyltransferase-2-bha17135353","title":"POMGNT2 Antibody \/ Protein O-linked mannose N-acetylglucosaminyltransferase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOMGNT2 Antibody \/ Protein O-linked mannose N-acetylglucosaminyltransferase 2 is a anti-POMGNT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POMGNT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOMGNT2 antibody detects Protein O-linked mannose N-acetylglucosaminyltransferase 2, an enzyme involved in the biosynthesis of O-mannosyl glycans essential for proper glycoprotein maturation. POMGNT2 catalyzes the addition of N-acetylglucosamine to O-linked mannose on target proteins, an essential step in forming the core M1 glycan structure. This modification plays a pivotal role in the development and stability of the nervous system and skeletal muscle by ensuring correct glycosylation of proteins such as alpha-dystroglycan. The POMGNT2 antibody is an important research tool for studying congenital muscular dystrophies and glycosylation disorders affecting the central nervous system and skeletal tissues.\u003cbr\u003e\u003cbr\u003ePOMGNT2 is encoded by the POMGNT2 gene located on human chromosome 3q26.1. The protein is localized primarily in the Golgi apparatus, where it functions as a type II membrane-bound glycosyltransferase. Structurally, POMGNT2 contains a luminal catalytic domain responsible for UDP-GlcNAc transfer and a short cytoplasmic N-terminal tail that directs subcellular localization. This enzyme works sequentially with other glycosyltransferases, including POMT1, POMT2, and POMGNT1, to synthesize complex O-mannosyl glycans critical for basement membrane integrity and synaptic architecture.\u003cbr\u003e\u003cbr\u003eThe POMGNT2 antibody is frequently used in biochemical and histological studies examining glycosylation pathways disrupted in congenital muscular dystrophy-dystroglycanopathy type B14 (MDDGB14). Loss-of-function mutations in POMGNT2 result in hypoglycosylated alpha-dystroglycan, leading to reduced laminin binding and severe developmental abnormalities, including brain malformations and muscular dystrophy. Western blot analysis with this antibody typically identifies a band near 70�75 kDa corresponding to the mature enzyme, while immunofluorescence demonstrates punctate perinuclear staining consistent with Golgi localization.\u003cbr\u003e\u003cbr\u003eResearch employing POMGNT2 knockout models has revealed its necessity for neuronal migration, synaptic organization, and muscle fiber stability. In addition to its structural roles, glycosylation defects mediated by POMGNT2 deficiency affect signal transduction and receptor clustering in developing tissues.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POMGNT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POMGNT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POMGNT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POMGNT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POMGNT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286628717,"sku":"FY12450","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d5824b39-aced-476d-add3-786e2f7523f8.jpg?v=1782236998","url":"https:\/\/www.ebiohippo.com\/products\/pomgnt2-antibody-protein-o-linked-mannose-n-acetylglucosaminyltransferase-2-bha17135353","provider":"BioHippo","version":"1.0","type":"link"}