{"product_id":"pomt1-antibody-protein-o-mannosyl-transferase-1-bha17136127","title":"POMT1 Antibody \/ Protein O-mannosyl-transferase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePOMT1 Antibody \/ Protein O-mannosyl-transferase 1 is a anti-POMT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e POMT1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePOMT1 antibody detects Protein O-mannosyl-transferase 1, an enzyme required for the initial step of O-mannosyl glycan biosynthesis on glycoproteins, particularly alpha-dystroglycan. The UniProt recommended name is Protein O-mannosyl-transferase 1 (POMT1). This enzyme catalyzes the transfer of mannose from dolichol phosphate-mannose to serine or threonine residues on target proteins, a process essential for proper protein folding, stability, and function.\u003cbr\u003e\u003cbr\u003eFunctionally, POMT1 antibody identifies a 747-amino-acid endoplasmic reticulum (ER) membrane protein that forms a functional heterodimer with POMT2. This complex initiates the O-mannosyl glycosylation pathway, which is critical for dystroglycan processing and muscle integrity. POMT1 ensures the attachment of the first mannose residue, forming the foundation for subsequent glycan extensions that link the extracellular matrix to the cytoskeleton via alpha-dystroglycan.\u003cbr\u003e\u003cbr\u003eThe POMT1 gene is located on chromosome 9q34.13 and is expressed in brain, skeletal muscle, and heart. It plays a vital role in muscle development, neuronal migration, and synaptic organization by supporting glycosylation-dependent cell adhesion and signaling.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in POMT1 cause congenital muscular dystrophy-dystroglycanopathy type A1 (Walker-Warburg syndrome), type B1, and type C1. These disorders are characterized by muscular dystrophy, brain malformations, and ocular defects due to defective O-mannosylation of dystroglycan. Research using POMT1 antibody supports studies in glycosylation pathways, muscular dystrophy, and neuronal development.\u003cbr\u003e\u003cbr\u003ePOMT1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER glycosyltransferases. \u003cbr\u003e\u003cbr\u003eStructurally, Protein O-mannosyl-transferase 1 contains multiple transmembrane domains and a luminal catalytic domain harboring the conserved DXD motif required for glycosyltransferase activity. It assembles with POMT2 to form an enzymatically active complex that determines substrate recognition and catalysis. This antibody aids in investigating POMT1�s role in glycoprotein biosynthesis, muscle development, and congenital disease mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=POMT1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=POMT1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=POMT1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/POMT1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=POMT1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319003501,"sku":"FY13225","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fb03b69d-24a3-4ba6-97a5-827241154d86.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/pomt1-antibody-protein-o-mannosyl-transferase-1-bha17136127","provider":"BioHippo","version":"1.0","type":"link"}