{"product_id":"porcine-jev-antibody-elisa-kit-bhe21400563","title":"Porcine JEV Antibody ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Porcine JEV Antibody ELISA Kit is an indirect ELISA kit for quantitative measurement of porcine antibodies against JEV in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003cp\u003eIn this indirect format, porcine antibodies against JEV in the sample bind in the pre-coated wells and are then detected with an enzyme-labeled reagent, so the optical density rises with the antibody level. Results are calculated from the standard curve run on the same plate.\u003c\/p\u003e\n\u003cp\u003eValues are expressed relative to the antibody standard supplied with the kit.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eIndirect (Quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.25 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56 - 100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003e80-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eELISA Microtiter plate\u003c\/td\u003e\n\u003ctd\u003e96 wells*2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate\u003c\/td\u003e\n\u003ctd\u003e24 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10×Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent A\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent B\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive Control\u003c\/td\u003e\n\u003ctd\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNegative Control\u003c\/td\u003e\n\u003ctd\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e3 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSealed Bag\u003c\/td\u003e\n\u003ctd\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure\u003c\/h2\u003e\n\u003cp\u003eBring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eNumber the sample and control in order (in duplicate), and keep a record of control wells and sample wells. Set 2 wells for negative\/positive control. Set 1 blank control well， Run samples in duplicate.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL of positive\/negative control to positive\/negative control well, add 100 µL of Sample Diluent to the blank well, and add 100 µL of diluted serum to the sample wells.\u003c\/li\u003e\n\u003cli\u003eCover with a plate sealer and mix thoroughly, incubate at 37 °C for 30 min protected from light.\u003c\/li\u003e\n\u003cli\u003eRemove the liquid in each well. Immediately add 350 µL of Wash Buffer to each well and wash. Repeat 3 times, soaking 60 s each time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL of HRP Conjugate to each well, cover with a plate sealer and incubate at 37 °C for 30 min protected from light.\u003c\/li\u003e\n\u003cli\u003eRepeat step 4 for washing.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well. (Add Substrate Reagent A first and then Substrate Reagent B, please add in order) Cover with a plate sealer and mix thoroughly, incubate at 37 °C for 10 min protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well, mix thoroughly.\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance of each well by using a microplate reader with 450 nm (it is recommended to set the dual wavelength at 450 nm\/630 nm) wavelength.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRequired but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm \/ 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.\u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. Store the kit at 2-8 °C and do not freeze. Once opened, keep the plate at 2-8 °C and use it within 1 month. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980405932397,"sku":"VK489018-96T","price":1226.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_93268a7d-1ed3-47db-b6ad-9f62464202db.jpg?v=1794521012","url":"https:\/\/www.ebiohippo.com\/products\/porcine-jev-antibody-elisa-kit-bhe21400563","provider":"BioHippo","version":"1.0","type":"link"}