{"product_id":"porcine-taenia-solium-igg-elisa-kit-bhe21400546","title":"Porcine Taenia Solium IgG ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Porcine Taenia Solium IgG ELISA Kit quantifies porcine IgG antibodies against Taenia Solium in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003cp\u003eValues are expressed relative to the antibody standard supplied with the kit.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eQuantitative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.25 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56 - 100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003e80-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eELISA Microtiter plate\u003c\/td\u003e\n\u003ctd\u003e96 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilution plate\u003c\/td\u003e\n\u003ctd\u003e96 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20×Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent A\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent B\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive Control\u003c\/td\u003e\n\u003ctd\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNegative Control\u003c\/td\u003e\n\u003ctd\u003e0.5mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e3 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSealed Bag\u003c\/td\u003e\n\u003ctd\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure\u003c\/h2\u003e\n\u003cp\u003eBring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eRemove the required number of strips from the foil pouch after equilibration to room temperature for 20 minutes. Return unused strips to the resealable pouch and store at 4 °C.\u003c\/li\u003e\n\u003cli\u003eSet negative control wells, positive control wells, and sample wells. Add 50 µL of negative control and positive control to their respective wells.\u003c\/li\u003e\n\u003cli\u003eFor test sample wells, first add 10 µL of the test sample, then add 40 µL of sample diluent.\u003c\/li\u003e\n\u003cli\u003eThen add 100 µL of HRP labelled detection antibody to each well (including control and sample wells). Cover the plate with the sealing film and incubate at 37 °C for 60 minutes in a water bath or incubator.\u003c\/li\u003e\n\u003cli\u003eDiscard the liquid, tap dry on absorbent paper. Fill each well with wash buffer, let stand for 1 minute, discard the wash buffer, and tap dry on absorbent paper. Repeat the wash 5 times (an automatic plate washer may also be used).\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Substrate A and 50 µL of Substrate B to each well. Incubate at 37 °C in the dark for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well. Measure the OD of each well at 450 nm within 15 minutes.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRequired but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm \/ 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.\u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. Store the kit at 2-8 °C and do not freeze. Once opened, keep the plate at 2-8 °C and use it within 1 month. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980403999085,"sku":"JN589018-96T","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_50f42e11-4891-41e3-8cc3-9cdef197e0c5.jpg?v=1794521011","url":"https:\/\/www.ebiohippo.com\/products\/porcine-taenia-solium-igg-elisa-kit-bhe21400546","provider":"BioHippo","version":"1.0","type":"link"}