{"product_id":"ppar-alpha-reporter-lentivirus-bhv19400068","title":"PPAR-alpha Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePeroxisome proliferator-activated receptor alpha (PPAR-alpha) is a ligand-activated nuclear receptor and a central regulator of lipid metabolism. Highly expressed in the liver, it is activated by fatty acids and their derivatives and heterodimerizes with the retinoid X receptor to bind PPAR response elements (PPRE) in target gene promoters. PPAR-alpha drives the transcription of genes controlling fatty acid uptake, mitochondrial and peroxisomal beta-oxidation, and ketogenesis, particularly during fasting. It is the molecular target of fibrate drugs used to lower circulating lipids. Because of its role in energy homeostasis and lipid handling, PPAR-alpha is an important focus of research into metabolic disorders, fatty liver disease, and diabetes.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PPAR-alpha Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PPAR-alpha activity in mammalian cells. The construct contains tandem repeats of the PPRE element from the Acox1 promoter placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. The design favors activation by PPAR-alpha and RXR-alpha heterodimers and responds more strongly to PPAR-alpha than to PPAR-gamma. A constitutive drug selection marker (Puromycin or Blasticidin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying PPAR signaling in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the PPARa transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251623977325,"sku":"LTV-0073-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310904926573,"sku":"LTV-0073-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310904959341,"sku":"LTV-0073-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310904992109,"sku":"LTV-0073-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310905024877,"sku":"LTV-0073-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310905057645,"sku":"LTV-0073-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0073-PPARa-Reporter-Fig1.jpg?v=1776934578","url":"https:\/\/www.ebiohippo.com\/products\/ppar-alpha-reporter-lentivirus-bhv19400068","provider":"BioHippo","version":"1.0","type":"link"}