{"product_id":"ppar-gamma-antibody-pparg-bha17135753","title":"PPAR gamma Antibody \/ PPARG","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPAR gamma Antibody \/ PPARG is a anti-PPAR Rabbit antibody Recombinant Rabbit Monoclonal clone 31P97 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPAR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31P97, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPAR gamma antibody targets peroxisome proliferator activated receptor gamma, a nuclear receptor encoded by the PPARG gene. PPAR gamma functions as a ligand activated transcription factor and plays essential roles in adipocyte differentiation, glucose metabolism, and lipid storage. It binds to specific DNA sequences as a heterodimer with RXR, regulating genes involved in insulin sensitivity, fatty acid uptake, and inflammatory responses.\u003cbr\u003e\u003cbr\u003ePPAR gamma exists in at least two isoforms, PPAR gamma 1 and PPAR gamma 2, which differ in tissue distribution and regulatory control. PPAR gamma 1 is broadly expressed, while PPAR gamma 2 is more adipocyte specific. Both isoforms are activated by endogenous ligands such as prostaglandin derivatives and polyunsaturated fatty acids, as well as synthetic ligands like thiazolidinediones. These synthetic agonists have been widely used as antidiabetic medications, making PPAR gamma antibody an important reagent in metabolic disease research.\u003cbr\u003e\u003cbr\u003eThe receptor is also implicated in cancer biology. PPAR gamma can regulate cell cycle arrest, apoptosis, and differentiation. Studies using PPAR gamma antibody have shown altered expression in breast, colon, and prostate cancers. Inflammation research has further demonstrated that PPAR gamma modulates immune responses by suppressing pro inflammatory cytokine production in macrophages and T cells.\u003cbr\u003e\u003cbr\u003eDetection of PPAR gamma with PPAR gamma antibody is routinely carried out in western blotting, immunohistochemistry, and chromatin immunoprecipitation. Western blotting reveals isoform specific expression, while immunohistochemistry highlights nuclear localization. Chromatin immunoprecipitation assays combined with next generation sequencing have uncovered PPAR gamma binding sites across the genome, providing insights into transcriptional networks controlling energy homeostasis.\u003cbr\u003e\u003cbr\u003ePPAR gamma remains a therapeutic target for type 2 diabetes, obesity, and cardiovascular disease. Researchers rely on PPAR gamma antibody to study receptor expression, ligand responsiveness, and downstream signaling changes under physiological and pathological conditions. Availability of validated antibodies such as those from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPAR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPAR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPAR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPAR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPAR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047304356205,"sku":"FY12851","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cdff9bb6-c03a-41bb-944a-e5f755a5f250.jpg?v=1772019352","url":"https:\/\/www.ebiohippo.com\/products\/ppar-gamma-antibody-pparg-bha17135753","provider":"BioHippo","version":"1.0","type":"link"}