{"product_id":"ppar-gamma-reporter-lentivirus-bhv19400027","title":"PPAR-gamma Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePeroxisome proliferator-activated receptor gamma (PPAR-gamma) is a ligand-activated nuclear receptor and a master regulator of adipocyte differentiation and lipid storage. Activated by fatty acid derivatives and synthetic agonists, PPAR-gamma heterodimerizes with the retinoid X receptor and binds PPAR response elements (PPRE) to control genes governing adipogenesis, glucose homeostasis, and insulin sensitivity. It is also expressed in macrophages and other cell types, where it modulates inflammatory responses. PPAR-gamma is the molecular target of thiazolidinedione drugs used to improve insulin sensitivity, and its activity is central to studies of obesity, type 2 diabetes, and metabolic disease, making it a key focus of metabolism and endocrinology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PPAR-gamma Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PPAR-gamma activity in transduced cells. The construct places a reporter gene (firefly luciferase, GFP, RFP, or Renilla luciferase) under the control of tandem PPAR response elements optimized for preferential activation by PPAR-gamma over the PPAR-alpha and PPAR-delta isoforms, coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying PPAR-gamma activation in primary and difficult-to-transfect cells for metabolism and endocrinology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251624010093,"sku":"LTV-0031-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310897291629,"sku":"LTV-0031-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310897324397,"sku":"LTV-0031-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897357165,"sku":"LTV-0031-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310897389933,"sku":"LTV-0031-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310897422701,"sku":"LTV-0031-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310897455469,"sku":"LTV-0031-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0031-PPRE-Reporter-PPARg-Fig1-1.jpg?v=1776934449","url":"https:\/\/www.ebiohippo.com\/products\/ppar-gamma-reporter-lentivirus-bhv19400027","provider":"BioHippo","version":"1.0","type":"link"}