{"product_id":"ppih-antibody-peptidyl-prolyl-cis-trans-isomerase-h-bha17135875","title":"PPIH Antibody \/ Peptidyl-prolyl cis-trans isomerase H","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPIH Antibody \/ Peptidyl-prolyl cis-trans isomerase H is a anti-PPIH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPIH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPIH antibody detects Peptidyl-prolyl cis-trans isomerase H, a cyclophilin family enzyme that assists in protein folding and pre-mRNA splicing. The UniProt recommended name is Peptidyl-prolyl cis-trans isomerase H (PPIH), also known as Cyclophilin H. PPIH catalyzes the cis-trans isomerization of peptide bonds preceding proline residues, a rate-limiting step in protein folding and conformational regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, PPIH antibody identifies a 177-amino-acid protein localized primarily in the nucleus, where it associates with the spliceosome machinery. PPIH is a component of the U4\/U6.U5 tri-snRNP complex, interacting with PRPF4, SNU13, and other splicing factors. Through its isomerase activity, PPIH facilitates conformational rearrangements necessary for spliceosome assembly and catalysis. Beyond its role in RNA splicing, PPIH also contributes to protein folding and trafficking, reflecting its general chaperone-like activity within the cyclophilin family.\u003cbr\u003e\u003cbr\u003eThe PPIH gene is located on chromosome 1p34.2 and encodes a protein containing a single cyclophilin-type peptidyl-prolyl isomerase (PPIase) domain. PPIH is structurally similar to other cyclophilins such as PPIA and PPIL1 but exhibits specialized interactions within the spliceosome. Its enzymatic activity accelerates folding of substrates containing cis-proline bonds, influencing the conformation of RNA-binding proteins and enzymes involved in gene expression.\u003cbr\u003e\u003cbr\u003eLoss or inhibition of PPIH function affects pre-mRNA splicing efficiency and fidelity, leading to transcriptome-wide alterations. Dysregulation of PPIH expression has been observed in cancer and neurodegenerative diseases, suggesting roles in RNA metabolism and stress response. In cell signaling, PPIH interacts with transcription and export factors, linking splicing to mRNA maturation and nuclear export.\u003cbr\u003e\u003cbr\u003ePPIH antibody is used to study RNA processing, protein folding, and chaperone function. It is applicable for immunoblotting, immunoprecipitation, and fluorescence microscopy to examine PPIH localization and protein-protein interactions. In structural biology, PPIH serves as a model enzyme for studying cyclophilin catalytic mechanisms. Its sensitivity to cyclosporine A highlights its functional conservation across cyclophilins and its potential involvement in immunomodulatory pathways.\u003cbr\u003e\u003cbr\u003eStructurally, PPIH features a conserved beta-barrel fold typical of cyclophilins, with a hydrophobic active site pocket that accommodates the proline-containing peptide bond. It is post-translationally regulated by phosphorylation and complex assembly within spliceosomal subunits.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPIH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPIH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPIH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPIH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPIH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307862381,"sku":"FY12973","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8a38ccc0-e44d-45bf-b70a-7f56ae8f02a5.jpg?v=1782237070","url":"https:\/\/www.ebiohippo.com\/products\/ppih-antibody-peptidyl-prolyl-cis-trans-isomerase-h-bha17135875","provider":"BioHippo","version":"1.0","type":"link"}