{"product_id":"ppm1g-antibody-protein-phosphatase-1g-bha17135915","title":"PPM1G Antibody \/ Protein phosphatase 1G","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPM1G Antibody \/ Protein phosphatase 1G is a anti-PPM1G Rabbit antibody Recombinant Rabbit Monoclonal clone 29P73 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPM1G\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29P73, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPM1G antibody detects Protein phosphatase 1G, encoded by the PPM1G gene. Protein phosphatase 1G belongs to the PP2C family of serine threonine phosphatases, which are magnesium or manganese dependent enzymes involved in negative regulation of cell signaling. PPM1G dephosphorylates specific substrates to modulate cell cycle progression, DNA damage repair, pre mRNA splicing, and chromatin remodeling. PPM1G antibody is a versatile reagent for exploring how phosphatase activity contributes to cellular regulation and disease.\u003cbr\u003e\u003cbr\u003eProtein phosphatase 1G has been implicated in regulation of the G2\/M transition of the cell cycle. Research using PPM1G antibody has shown that depletion of this phosphatase delays mitotic entry and leads to accumulation of phosphorylated cyclin dependent kinase substrates. By reversing phosphorylation of key regulators, PPM1G ensures proper timing of mitotic onset. Its involvement in cell cycle checkpoints makes it critical for maintaining genomic stability and preventing uncontrolled proliferation.\u003cbr\u003e\u003cbr\u003ePPM1G also plays a role in DNA damage responses. Studies using PPM1G antibody have demonstrated that it dephosphorylates histone H2AX and other chromatin associated substrates, facilitating repair and chromatin restoration following DNA double strand breaks. This highlights its function as a chromatin associated phosphatase linking signaling pathways to epigenetic control. Dysregulation of PPM1G expression or activity may impair DNA repair, contributing to mutagenesis and cancer susceptibility.\u003cbr\u003e\u003cbr\u003eIn addition to DNA repair and cell cycle regulation, PPM1G is involved in pre mRNA splicing. By dephosphorylating components of the spliceosome, it regulates splicing factor dynamics and mRNA maturation. Research with PPM1G antibody has revealed that it controls alternative splicing patterns and transcriptional responses, linking phosphatase activity to gene expression. This broadens its functional impact beyond canonical phosphatase roles and highlights its integration into multiple layers of gene regulation.\u003cbr\u003e\u003cbr\u003ePPM1G antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting demonstrates expression across proliferative tissues, while immunohistochemistry localizes the phosphatase in nuclei of dividing cells. Immunoprecipitation with PPM1G antibody isolates its complexes, enabling characterization of substrates and interaction partners. Together, these approaches make PPM1G antibody an important tool for studying signaling, genome maintenance, and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eBy supplying validated PPM1G antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPM1G - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPM1G - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPM1G - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPM1G - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPM1G+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308845421,"sku":"FY13013","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7f062e4c-ab43-4f95-9701-80fd427aad40.jpg?v=1772019376","url":"https:\/\/www.ebiohippo.com\/products\/ppm1g-antibody-protein-phosphatase-1g-bha17135915","provider":"BioHippo","version":"1.0","type":"link"}