{"product_id":"ppp1r13l-antibody-iaspp-protein-phosphatase-1-regulatory-subunit-13-like-bha17136178","title":"PPP1R13L Antibody \/ iASPP \/ Protein phosphatase 1 regulatory subunit 13 like","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPP1R13L Antibody \/ iASPP \/ Protein phosphatase 1 regulatory subunit 13 like is a anti-PPP1R13L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP1R13L\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPP1R13L antibody detects Protein phosphatase 1 regulatory subunit 13 like, a transcriptional regulator that interacts with p53, NF-kappaB, and protein phosphatase 1 (PP1) to control apoptosis and gene expression. The UniProt recommended name is Protein phosphatase 1 regulatory subunit 13 like (PPP1R13L). Also known as inhibitor of apoptosis-stimulating protein of p53 (iASPP), it acts as a negative regulator of p53-mediated transcription and apoptosis.\u003cbr\u003e\u003cbr\u003eFunctionally, PPP1R13L antibody identifies a 918-amino-acid nuclear and cytoplasmic protein that binds to the DNA-binding domain of p53, preventing the activation of pro-apoptotic genes such as BAX and PUMA. PPP1R13L also interacts with the RelA\/p65 subunit of NF-kappaB, inhibiting its transcriptional activity and modulating inflammatory signaling. Through its association with PP1, it influences the phosphorylation state of nuclear proteins, linking phosphatase activity to transcriptional repression.\u003cbr\u003e\u003cbr\u003eThe PPP1R13L gene is located on chromosome 19q13.32 and is expressed in multiple tissues, including heart, liver, and brain, with elevated expression in dividing cells and tumors. Expression is induced by growth factors and oncogenic signals, reflecting its role in balancing survival and stress responses. PPP1R13L is part of the ASPP (apoptosis-stimulating proteins of p53) family, which fine-tunes p53 function depending on cellular context.\u003cbr\u003e\u003cbr\u003ePathologically, overexpression of PPP1R13L contributes to oncogenesis by suppressing p53-dependent apoptosis and enhancing cell survival under stress. High levels are found in breast, liver, and hematologic malignancies. Conversely, reduced expression sensitizes cells to apoptosis. Genetic variants have also been linked to cardiomyopathy and developmental abnormalities. Research using PPP1R13L antibody supports studies in apoptosis regulation, tumor biology, and transcriptional control.\u003cbr\u003e\u003cbr\u003ePPP1R13L antibody can be validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcriptional regulators. \u003cbr\u003e\u003cbr\u003eStructurally, Protein phosphatase 1 regulatory subunit 13 like contains ankyrin repeats and an SH3 domain that mediate interactions with p53 and NF-kappaB, as well as PP1-binding motifs for phosphatase association. This modular structure allows PPP1R13L to serve as a multifunctional scaffold integrating transcriptional repression and survival signaling. This antibody supports investigation of PPP1R13L's role in cell fate determination, stress response, and tumor progression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPP1R13L - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP1R13L - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP1R13L - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPP1R13L - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP1R13L+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319789933,"sku":"FY13276","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ba39385a-4284-4715-ac5d-5e09b5edbef5.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/ppp1r13l-antibody-iaspp-protein-phosphatase-1-regulatory-subunit-13-like-bha17136178","provider":"BioHippo","version":"1.0","type":"link"}