{"product_id":"ppp1r8-antibody-protein-phosphatase-1-regulatory-subunit-8-bha17135360","title":"PPP1R8 Antibody \/ Protein phosphatase 1 regulatory subunit 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPP1R8 Antibody \/ Protein phosphatase 1 regulatory subunit 8 is a anti-PPP1R8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP1R8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPP1R8 antibody detects Protein phosphatase 1 regulatory subunit 8, also known as Nuclear inhibitor of protein phosphatase-1 (NIPP1), a multifunctional nuclear protein that regulates the activity of protein phosphatase 1 (PP1). PPP1R8 modulates pre-mRNA splicing, transcription, and DNA damage responses through its interaction with PP1 catalytic subunits and RNA-binding proteins. The PPP1R8 antibody is a valuable tool for investigating phosphatase regulation, RNA processing, and nuclear signaling pathways associated with gene expression and chromatin remodeling.\u003cbr\u003e\u003cbr\u003ePPP1R8 is encoded by the PPP1R8 gene on human chromosome 1p35.1. The protein contains a central PP1-binding motif (RVXF) that mediates association with PP1, a forkhead-associated (FHA) domain involved in phosphoprotein recognition, and a nuclear localization signal. PPP1R8 functions as both an inhibitor and targeting subunit of PP1, directing its activity toward specific nuclear substrates such as splicing factors and transcriptional regulators. It also participates in the regulation of cell cycle progression and DNA replication by modulating the phosphorylation state of histones and replication proteins.\u003cbr\u003e\u003cbr\u003eStudies using the PPP1R8 antibody have shown that the protein localizes predominantly to the nucleus, with strong accumulation in speckle-like structures associated with RNA splicing. Western blot analysis typically detects bands near 35-40 kDa corresponding to the primary isoform, although multiple splice variants exist. PPP1R8 interacts with CDC5L, SNW1, and other components of the spliceosome complex, underscoring its integral role in pre-mRNA processing. In addition to RNA metabolism, PPP1R8 regulates transcription by interacting with Polycomb repressive complex proteins and influencing histone methylation states.\u003cbr\u003e\u003cbr\u003eFunctionally, PPP1R8 acts as a checkpoint in cellular stress responses and may modulate signaling cascades involving Akt and MAP kinases. Dysregulation has been implicated in cancer and neurodegenerative disorders through its impact on protein phosphorylation balance and gene expression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPP1R8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP1R8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP1R8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPP1R8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP1R8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286530413,"sku":"FY12457","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cacdfc57-0d0e-45d4-875d-2cde945a2a24.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/ppp1r8-antibody-protein-phosphatase-1-regulatory-subunit-8-bha17135360","provider":"BioHippo","version":"1.0","type":"link"}