{"product_id":"ppp2r5d-antibody-protein-phosphatase-2-regulatory-subunit-b-delta-bha17135652","title":"PPP2R5D Antibody \/ Protein phosphatase 2 regulatory subunit B delta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPP2R5D Antibody \/ Protein phosphatase 2 regulatory subunit B delta is a anti-PPP2R5D Rabbit antibody Recombinant Rabbit Monoclonal clone 30P07 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP2R5D\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P07, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPP2R5D antibody detects protein phosphatase 2 regulatory subunit B delta, encoded by the PPP2R5D gene. This protein is also known as PP2A B56 delta, phosphatase 2A regulatory subunit PPP2R5D, and serine threonine phosphatase 2A B56 delta. PP2A is one of the major cellular serine threonine phosphatases, regulating cell cycle progression, DNA replication, apoptosis, and signal transduction. The PP2A holoenzyme is composed of a catalytic C subunit, a scaffolding A subunit, and a regulatory B subunit. PPP2R5D belongs to the B56 family of regulatory subunits that direct PP2A activity toward specific substrates and cellular compartments.\u003cbr\u003e\u003cbr\u003ePPP2R5D antibody is widely applied in neuroscience, developmental biology, and cancer research. Mutations in PPP2R5D cause neurodevelopmental disorders characterized by intellectual disability, hypotonia, macrocephaly, and autism spectrum features. By detecting PPP2R5D, researchers can study how alterations in PP2A signaling contribute to brain development and cognitive function. In cancer biology, PP2A acts as a tumor suppressor, and dysregulation of its regulatory subunits can promote oncogenesis.\u003cbr\u003e\u003cbr\u003ePPP2R5D directs PP2A activity toward key regulators such as Akt, MAPK, and MYC, influencing pathways that control proliferation and apoptosis. The antibody supports studies into how these signaling cascades are altered by mutations or changes in expression. Detection of PPP2R5D provides insight into phosphatase signaling networks that are otherwise difficult to measure directly.\u003cbr\u003e\u003cbr\u003e Western blot assays detect PPP2R5D protein in neuronal and cancer cell lysates. Immunohistochemistry maps tissue expression in brain, liver, and tumors. Immunofluorescence highlights nuclear or cytoplasmic localization depending on signaling context. These tools provide comprehensive approaches for studying PP2A regulation.\u003cbr\u003e\u003cbr\u003ePPP2R5D is implicated in rare genetic disorders grouped as PPP2R5D related neurodevelopmental syndromes. These conditions result from de novo mutations and highlight the critical role of PP2A signaling in neurodevelopment. By using PPP2R5D antibody, scientists can explore genotype phenotype correlations and mechanisms underlying disease pathology.\u003cbr\u003e\u003cbr\u003eIn oncology, dysregulated PP2A contributes to leukemia, breast cancer, and colorectal cancer. Altered expression of regulatory subunits like PPP2R5D changes phosphatase balance, leading to sustained oncogenic signaling. Therapeutic strategies are exploring reactivation of PP2A complexes as a tumor suppressor approach. PPP2R5D antibody therefore supports both mechanistic studies and translational drug development.\u003cbr\u003e\u003cbr\u003ePPP2R5D antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPP2R5D - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP2R5D - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP2R5D - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPP2R5D - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP2R5D+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047299080557,"sku":"FY12749","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8193fcb7-4ac9-40ac-828d-d94bc0aeac7d.jpg?v=1772019338","url":"https:\/\/www.ebiohippo.com\/products\/ppp2r5d-antibody-protein-phosphatase-2-regulatory-subunit-b-delta-bha17135652","provider":"BioHippo","version":"1.0","type":"link"}