{"product_id":"ppp2r5e-antibody-serine-threonine-protein-phosphatase-2a-regulatory-subunit-b-epsilon-bha17135947","title":"PPP2R5E Antibody \/ Serine threonine protein phosphatase 2A regulatory subunit B epsilon","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePPP2R5E Antibody \/ Serine threonine protein phosphatase 2A regulatory subunit B epsilon is a anti-PPP2R5E Rabbit antibody Recombinant Rabbit Monoclonal clone 30P58 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP2R5E\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P58, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePPP2R5E antibody detects Serine threonine protein phosphatase 2A regulatory subunit B epsilon, encoded by the PPP2R5E gene. Protein phosphatase 2A is one of the major serine threonine phosphatases in eukaryotic cells, controlling phosphorylation dependent signaling across pathways that regulate cell cycle progression, apoptosis, and metabolism. PPP2R5E belongs to the B56 family of regulatory subunits, which determine substrate specificity and subcellular localization of PP2A holoenzymes. PPP2R5E antibody provides a powerful reagent for investigating the diverse roles of this phosphatase in physiology and disease.\u003cbr\u003e\u003cbr\u003ePP2A holoenzymes consist of a scaffolding subunit, a catalytic subunit, and one of multiple regulatory subunits. PPP2R5E directs PP2A activity to specific substrates at centrosomes, kinetochores, and other cellular structures. Research using PPP2R5E antibody has shown that this regulatory subunit influences chromosome segregation, spindle formation, and checkpoint activation. These roles make it critical in maintaining genome stability and proper mitotic progression.\u003cbr\u003e\u003cbr\u003eIn cancer biology, PP2A acts as a tumor suppressor, and its inactivation contributes to oncogenic signaling. Altered expression of regulatory subunits such as PPP2R5E can impair PP2A function, enhancing signaling through pathways such as PI3K AKT and MAPK. Research with PPP2R5E antibody has demonstrated that loss of this subunit promotes tumorigenesis and contributes to chemotherapy resistance. Conversely, restoration of PP2A activity has been explored as a therapeutic strategy in malignancy, highlighting the importance of PPP2R5E in cancer control.\u003cbr\u003e\u003cbr\u003eBeyond cancer, PPP2R5E has roles in neurodegeneration and cardiovascular disease. Studies using PPP2R5E antibody have linked PP2A dysfunction to tau hyperphosphorylation and Alzheimer disease pathology. In the cardiovascular system, PP2A regulatory subunits contribute to signaling in heart muscle contraction and vascular function. These findings underscore the broad physiological impact of PPP2R5E.\u003cbr\u003e\u003cbr\u003ePPP2R5E antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting measures expression across proliferative and neuronal tissues, immunohistochemistry highlights nuclear localization, and immunoprecipitation isolates PP2A complexes containing PPP2R5E. These applications demonstrate the versatility of PPP2R5E antibody in molecular biology and translational research.\u003cbr\u003e\u003cbr\u003eBy supplying validated PPP2R5E antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PPP2R5E - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP2R5E - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP2R5E - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PPP2R5E - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP2R5E+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047310057837,"sku":"FY13045","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ebfcbca4-7890-4df0-84e1-3963b35f18ad.jpg?v=1772019383","url":"https:\/\/www.ebiohippo.com\/products\/ppp2r5e-antibody-serine-threonine-protein-phosphatase-2a-regulatory-subunit-b-epsilon-bha17135947","provider":"BioHippo","version":"1.0","type":"link"}