{"product_id":"prag1-antibody-pragmin-peak1-related-kinase-activating-pseudokinase-1-bha17135766","title":"PRAG1 Antibody \/ Pragmin \/ PEAK1-related kinase-activating pseudokinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRAG1 Antibody \/ Pragmin \/ PEAK1-related kinase-activating pseudokinase 1 is a anti-PRAG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nucleus, Cell Junctions, possible Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRAG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRAG1 antibody detects PEAK1-related, actin-binding guanine nucleotide exchange factor, also called Pragmin, a cytoskeletal and signaling adaptor involved in focal adhesion dynamics, actin organization, and cell migration. Encoded by the PRAG1 gene on chromosome 8q24.3, this multidomain protein acts as a nonreceptor tyrosine kinase scaffold that integrates signals from growth factor receptors and integrins to regulate cytoskeletal remodeling and motility. PRAG1 plays an important role in developmental morphogenesis and cancer cell invasion.\u003cbr\u003e\u003cbr\u003eStructurally, PRAG1 contains an N-terminal actin-binding domain, a proline-rich region that interacts with SH3 domain-containing proteins, and multiple tyrosine phosphorylation motifs that recruit Src family kinases. Although it lacks intrinsic kinase activity, PRAG1 serves as a platform that enhances activation of FAK, Src, and downstream MAPK signaling, linking mechanical and biochemical cues during adhesion turnover. Its localization to focal adhesions and actin stress fibers reflects its role in maintaining cellular structure and migration capacity.\u003cbr\u003e\u003cbr\u003eThe PRAG1 antibody is widely used in cancer, developmental biology, and cytoskeletal signaling research to investigate adhesion signaling, actin dynamics, and metastatic potential. Western blot analysis detects a 170 kilodalton band corresponding to PRAG1, while immunofluorescence shows peripheral staining at focal adhesion sites and along actin filaments. This antibody supports studies examining integrin-mediated signaling and tumor cell invasion mechanisms.\u003cbr\u003e\u003cbr\u003eAberrant expression of PRAG1 contributes to epithelial-mesenchymal transition (EMT), tumor metastasis, and angiogenesis. Elevated PRAG1 levels enhance migration and invasion by promoting FAK\/Src complex activation and actin remodeling. Conversely, depletion of PRAG1 suppresses motility and reduces metastatic spread. The PRAG1 antibody provides an essential tool for dissecting these molecular pathways and evaluating PRAG1 as a potential therapeutic target in oncology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRAG1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRAG1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRAG1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRAG1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRAG1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304716653,"sku":"FY12864","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a03a737a-c301-48e2-8487-2dac57881a8b.jpg?v=1782237049","url":"https:\/\/www.ebiohippo.com\/products\/prag1-antibody-pragmin-peak1-related-kinase-activating-pseudokinase-1-bha17135766","provider":"BioHippo","version":"1.0","type":"link"}