{"product_id":"pras40-blocking-peptide-stj505498-bhp15502022","title":"PRAS40 Blocking Peptide (STJ505498)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003ePRAS40 (Proline-rich AKT1 substrate 1, \u003cem\u003eAKT1S1\u003c\/em\u003e) is a direct AKT substrate and an inhibitory component of mTORC1. AKT-mediated phosphorylation of PRAS40 at Thr246 relieves mTORC1 inhibition, linking PI3K\/AKT signalling to downstream mTOR-dependent cell growth, protein synthesis, and autophagy. PRAS40 is a key node in oncogenic PI3K–AKT–mTOR signalling.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBlocking \/ Competition Peptide\u003c\/span\u003e\n\u003cp\u003eThis synthetic peptide corresponds to the immunogen sequence used to raise the matched St John's Laboratory antibody. It is supplied for immunodepletion and immunocompetition experiments to confirm antibody binding specificity. Pre-incubating the antibody with this peptide before immunoassay use abolishes or substantially reduces target-specific signal.\u003c\/p\u003e\n\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n  \u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eQ96B36\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eImmunogen\u003c\/th\u003e\n\u003ctd\u003eSynthetic peptide taken within amino acid region 160-210 on human Proline-rich AKT1 substrate 1 isoform a protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eImmunogen Region (aa)\u003c\/th\u003e\n\u003ctd\u003e160-210\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLiquid form at 2.5mg\/ml concentration in PBS. Up to 5% DMSO can be added. Orders with \u0026gt;1mg can be supplied in lyophilized powder form, or in buffer of choice.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at-20°C for long term storage. Avoid freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eImmunodepletion, Immunocompetition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eRecommended Protocol\u003c\/h2\u003e\n\u003cp\u003eIncubate the paired antibody (at working concentration) with a 5–10 molar excess of this peptide for 30–60 minutes at 4°C or room temperature. Centrifuge briefly if any precipitate forms, then use the pre-absorbed antibody in parallel with an unblocked control in the same experiment. Abolition or substantial reduction of signal confirms antibody specificity to the target epitope.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"250 ug","offer_id":53326596571501,"sku":"STJ505498-250","price":359.49,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/product-no-image_0cae1349-2a30-4b4a-b6fc-cb38dcdf34f6.png?v=1778704892","url":"https:\/\/www.ebiohippo.com\/products\/pras40-blocking-peptide-stj505498-bhp15502022","provider":"BioHippo","version":"1.0","type":"link"}