{"product_id":"prdm11-antibody-pr-domain-containing-protein-11-bha17136261","title":"PRDM11 Antibody \/ PR domain-containing protein 11","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRDM11 Antibody \/ PR domain-containing protein 11 is a anti-PRDM11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRDM11\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRDM11 antibody detects PR domain-containing protein 11, a nuclear transcriptional regulator encoded by the PRDM11 gene located on chromosome 11q24.2. PRDM11 belongs to the PR domain zinc finger protein family, known for roles in transcriptional repression, chromatin modification, and developmental gene regulation. It contains a PR (positive regulatory) domain with methyltransferase-related function and multiple C2H2-type zinc finger motifs for DNA binding. PRDM11 is expressed in lymphoid tissues, brain, and testis, where it influences lineage specification and cellular differentiation through epigenetic control.\u003cbr\u003e\u003cbr\u003ePRDM11 functions as a transcriptional repressor that modulates gene expression by recruiting histone-modifying enzymes such as histone methyltransferases and HDACs. Through these interactions, PRDM11 establishes repressive chromatin environments to silence developmental regulators. It plays a role in maintaining proper differentiation states of B cells and neurons by controlling genes involved in proliferation and signaling. Co-localization studies show PRDM11 concentrated in nuclear foci where transcriptional repression occurs.\u003cbr\u003e\u003cbr\u003eStructurally, PRDM11 contains an N-terminal PR\/SET domain that is homologous to histone lysine methyltransferases, although its intrinsic enzymatic activity remains unclear. The C-terminal region includes multiple zinc finger motifs that recognize specific DNA sequences and allow recruitment of co-repressors. It belongs to the PRDM family of transcriptional regulators, which includes PRDM1 (BLIMP-1), PRDM2, and PRDM16, all of which play pivotal roles in development and oncogenesis.\u003cbr\u003e\u003cbr\u003eFunctionally, PRDM11 contributes to chromatin-mediated gene silencing and developmental programming. It represses target genes involved in cell proliferation and signaling pathways, promoting stable cell identity. In neural tissues, PRDM11 is implicated in neuronal subtype specification, while in hematopoietic cells it influences differentiation and germinal center responses. It may also coordinate cross-talk between chromatin remodeling complexes and transcriptional repressors such as REST and HDAC1.\u003cbr\u003e\u003cbr\u003eDysregulation of PRDM11 is associated with lymphoma and other cancers. Loss of expression or chromosomal rearrangements involving PRDM11 are reported in diffuse large B-cell lymphoma, suggesting a tumor suppressor function. Aberrant PRDM11 activity can also disrupt neural differentiation or germ cell maturation. Pathway associations include chromatin organization, histone modification, and transcriptional repression networks. During embryogenesis, PRDM11 expression correlates with organogenesis and tissue patterning.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using PRDM11 antibody reveals nuclear localization in lymphocytes and neuronal cells. The PRDM11 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRDM11 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRDM11 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRDM11 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRDM11 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRDM11+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323165037,"sku":"FY13359","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_46273f76-f01b-4418-a63d-4de676969166.jpg?v=1782237104","url":"https:\/\/www.ebiohippo.com\/products\/prdm11-antibody-pr-domain-containing-protein-11-bha17136261","provider":"BioHippo","version":"1.0","type":"link"}