{"product_id":"premium-t4-dna-ligase-400-u-mu-l-bhz20800112","title":"Premium T4 DNA Ligase (400 U\/μL)","description":"\u003cp align=\"justify\"\u003eT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl termini of adjacent nucleotides in double-stranded DNA or RNA with either cohesive or blunt ends. This reaction requires ATP as a cofactor. Additionally, T4 DNA Ligase can repair single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA\/RNA hybrids.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"9\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eCat.No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"464\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e14966ES40 \/ 14966ES42\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"464\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e40 KU \/ 400 KU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eConcentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"464\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e400 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"464\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eRecombinant E. coli strain carrying the T4 DNA ligase gene.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"356\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eEnzyme Activity Definition\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"464\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eOne unit is defined as the amount of DNA ligase required to achieve 50% ligation of 100 ng of cohesive-end DNA fragments in 50 µL of 1X DNA Ligase Reaction Buffer with incubation for 30 minutes at 23°C.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eExcellent Heat Stability:\u003c\/strong\u003e Maintains over 60% residual activity after 4 hours at 42°C.\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eUltra-Low Adapter Self-Ligation:\u003c\/strong\u003e Cleaner Libraries, Higher Sensitivity\u003c\/li\u003e\n\u003cli align=\"justify\" class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eExcellent batch-to-batch consistency: \u003c\/strong\u003eStable and uniform performance.\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"9\" class=\"MsoNormalTable\" style=\"width: 99.9112%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.5613%;\" valign=\"center\" width=\"161\"\u003e\n\u003cp align=\"justify\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 40.1723%;\" valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\"\u003e14966ES40\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"top\" width=\"165\"\u003e\n\u003cp align=\"justify\"\u003e14966ES42\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.5613%;\" valign=\"top\" width=\"161\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e14966-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 40.1723%;\" valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003ePremium T4 DNA Ligase (400 U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e100 µL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"165\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.5613%;\" valign=\"top\" width=\"161\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14966-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 40.1723%;\" valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e2 × Rapid Ligation Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"165\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 24.5613%;\" valign=\"top\" width=\"161\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e14966-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 40.1723%;\" valign=\"center\" width=\"330\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10 × T4 DNA Ligase Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"149\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7784%;\" valign=\"center\" width=\"165\"\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp align=\"justify\"\u003e[Note]:\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e① High-concentration Premium T4 DNA Ligase combined with 2× Rapid Ligation Buffer significantly enhances the speed and efficiency of blunt-end ligation. Therefore, prolonged incubation (\u0026gt;10 minutes) is not recommended, as it may substantially reduce the transformation efficiency of the ligation products.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e② The 10× T4 DNA Ligase Buffer does not contain PEG and is compatible with standard ligation protocols that do not specify the use of rapid ligation buffer.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\"\u003eRoutine molecular cloning\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eHigh-throughput or automated library prep\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eLow-input NGS applications\u003c\/li\u003e\n\u003cli align=\"justify\"\u003ecfDNA and liquid biopsy workflows\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eChallenging ligation reactions requiring long incubation\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eProtocols involving room-temperature operations\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eWhole-genome library construction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 align=\"justify\"\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eExceptional Thermostability: Retains \u0026gt;60% Activity After 4 Hours at 42°C\u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 1. Thermostability assessment at 42°C and 45°C\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/CBCB71C1-4B0C-4c03-82B3-AE2F7B40DD17_1024x1024.png?v=1764120234\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 1. Thermostability assessment at 42°C and 45°C\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eYeasen Premium T4 DNA Ligase (Lots 1–3) and other suppliers were incubated at 42°C and 45°C for up to 4 hours. Activity was measured using a whole-genome library prep workflow with 1 µg bovine DNA. Yeasen ligase retained \u0026gt;60% activity at 42°C and outperformed competitors at 45°C, demonstrating superior thermostability.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eUltra-Low Adapter Dimerization: \u003c\/strong\u003eMinimal Self-Ligation with 0.5 ng gDNA in Library Prep\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 2. Comparison of adapter dimerization rates\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_4fff02d3-e077-4532-8d81-584d39bcc533_1024x1024.png?v=1764120273\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 2. Comparison of adapter dimerization rates\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThree batches of Yeasen Premium T4 DNA Ligase were compared with ligases from Supplier A and Supplier B using whole-genome library prep with 0.5 ng bovine DNA (Yeasen DNA Library Prep Kit, Cat# 12927ES). Yeasen ligase showed significantly lower adapter dimer formation, reducing non-specific ligation and improving usable sequencing yield.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eLow DNA Residuals\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 3. Host gDNA residual detection results for Premium T4 DNA Ligase.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_8d74815e-c2c9-471c-b70e-e178d622779c_1024x1024.png?v=1764120244\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 3. Host gDNA residual detection results for Premium T4 DNA Ligase.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThree batches of Yeasen Premium T4 DNA Ligase were tested for E. coli host gDNA contamination using the Yeasen E. coli Host Cell DNA Residual Detection Kit (Cat# 41318). All batches exhibited extremely low residual gDNA, well below the levels of imported brands, demonstrating high enzyme purity and ensuring reliable experimental outcomes.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e4. \u003c!--[endif]--\u003eNo Nuclease Contamination\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 4. Nuclease Contamination Test of Premium T4 DNA Ligase.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_3e6f3fff-6941-473c-8446-f5737713eded_1024x1024.png?v=1764120246\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 4. Nuclease Contamination Test of Premium T4 DNA Ligase.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThree batches of Premium T4 DNA Ligase (6000 U each) were incubated with nucleic acid substrates and analyzed by agarose gel electrophoresis. No exonuclease or nicking activity was detected, confirming the absence of nuclease contamination and ensuring sample integrity.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e5. \u003c!--[endif]--\u003eHigh Ligation Efficiency\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 5. Ligation-Mediated Circularization Efficiency Comparison.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_6e8e49fc-6819-4f1b-9a0d-ca310cf6763f_1024x1024.png?v=1764120247\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 5. Ligation-Mediated Circularization Efficiency Comparison.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003eThe circularization efficiency of Yeasen Premium T4 DNA Ligase (Cat# 14966) was compared with a conventional T4 DNA ligase(Cat#10301,Yeasen) using MGI libraries with either single-end or dual-index adapters. Circular products were pooled at equal mass and sequenced on the T7 sequencer. Yeasen 14966 consistently showed higher circularization efficiency and greater sequencing data yield than the conventional ligase.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e1. \u003c!--[endif]--\u003eFor your safety and health, wear a lab coat and disposable gloves while handling this product.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e2. \u003c!--[endif]--\u003eThis product is intended for research use only.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e3. \u003c!--[endif]--\u003eIt is normal for a small amount of precipitate to appear when the buffer is thawed. Simply invert and mix well before use.\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"40 KU","offer_id":53294150484333,"sku":"14966ES40","price":105.0,"currency_code":"USD","in_stock":true},{"title":"400 KU","offer_id":53294156611949,"sku":"14966ES42","price":805.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CBCB71C1-4B0C-4c03-82B3-AE2F7B40DD17_1024x1024_7c28ae5e-f9dd-4b8c-be7f-62c649cec269.png?v=1778101062","url":"https:\/\/www.ebiohippo.com\/products\/premium-t4-dna-ligase-400-u-mu-l-bhz20800112","provider":"BioHippo","version":"1.0","type":"link"}