{"product_id":"prepl-antibody-prolyl-endopeptidase-like-protein-bha17135904","title":"PREPL Antibody \/ Prolyl endopeptidase-like protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePREPL Antibody \/ Prolyl endopeptidase-like protein is a anti-PREPL Rabbit antibody Recombinant Rabbit Monoclonal clone 30P79 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PREPL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P79, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePREPL antibody detects Prolyl endopeptidase-like protein, encoded by the PREPL gene. This protein belongs to the prolyl oligopeptidase family of serine peptidases, but unlike many of its relatives, PREPL is thought to have limited or absent catalytic activity. Instead, it is believed to function as a scaffolding or regulatory protein influencing peptide processing, vesicle trafficking, and synaptic transmission. PREPL antibody provides an important reagent for studying rare genetic disease, peptide metabolism, and neurological function.\u003cbr\u003e\u003cbr\u003eMutations in PREPL cause a rare genetic disorder known as hypotonia-cystinuria syndrome, characterized by neonatal hypotonia, muscle weakness, and cystinuria due to loss of function in both PREPL and SLC3A1. Research using PREPL antibody has revealed that absence of Prolyl endopeptidase-like protein disrupts normal vesicle trafficking and neurotransmitter release, contributing to hypotonia and neurological deficits. Although the precise enzymatic role of PREPL remains unclear, its presence in synaptic vesicles suggests it contributes to regulated peptide handling and signaling in the nervous system.\u003cbr\u003e\u003cbr\u003eBeyond hypotonia-cystinuria syndrome, alterations in PREPL expression have been studied in broader contexts such as growth, appetite regulation, and endocrine function. Research with PREPL antibody has demonstrated its expression in brain and endocrine tissues, where it may modulate hormone release and metabolic signaling. Some studies suggest that PREPL deficiency influences growth hormone secretion, highlighting its potential role in development. These findings make PREPL an interesting target in both rare disease and general physiology.\u003cbr\u003e\u003cbr\u003ePREPL antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates tissue specific expression, especially in brain and muscle. Immunohistochemistry highlights its distribution in endocrine and neuronal tissues, while immunofluorescence confirms subcellular localization to vesicular structures. Together, these methods provide insights into PREPL's role in vesicle biology and signaling. Functional studies supported by PREPL antibody include knockout models where absence of PREPL reveals physiological consequences at cellular and organismal levels.\u003cbr\u003e\u003cbr\u003eBy supplying validated PREPL antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PREPL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PREPL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PREPL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PREPL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PREPL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308681581,"sku":"FY13002","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_015c8551-5398-4922-a043-b468299ebf50.jpg?v=1772019374","url":"https:\/\/www.ebiohippo.com\/products\/prepl-antibody-prolyl-endopeptidase-like-protein-bha17135904","provider":"BioHippo","version":"1.0","type":"link"}