{"product_id":"primary-mouse-c57bi-6-hepatocytes-plateable-bhc10901835","title":"Primary Mouse (C57BI\/6) Hepatocytes, Plateable","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePrimary Mouse (C57BI\/6) Hepatocytes, Plateable are mouse frozen primary cells from liver used for drug metabolism, hepatotoxicity, liver function, and cell-based assay development. These cells display adherent, polygonal growth characteristics.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Mouse (M. musculus)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Liver\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDonor history:\u003c\/strong\u003e Normal tissue\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0122) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eHepatocytes are central to xenobiotic metabolism, intermediary metabolism, protein synthesis, and bile-related functions. Primary hepatocyte models are widely used when physiologic metabolic capacity is important.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary hepatocyte cultures remain important for metabolism, transporter, and toxicity studies requiring liver-relevant function.\u003c\/li\u003e\n\u003cli\u003eLonger-term maintenance and co-culture approaches are used to preserve phenotype for repeated-dose or chronic-exposure designs.\u003c\/li\u003e\n\u003cli\u003eResearchers often profile donor-to-donor variability in metabolism and inducible response pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eEvaluate metabolism, transporter activity, or toxicity responses in liver-relevant in vitro systems.\u003c\/li\u003e\n\u003cli\u003eMeasure inducible gene expression or secreted markers after compound or cytokine treatment.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture workflows to model hepatic crosstalk with stromal or immune cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0122) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901835\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195751653741,"sku":"T0122","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/JNeg04r92iR20yjQzbJhOblY1R5VJ3kfQPlW8L5R.png?v=1775322481","url":"https:\/\/www.ebiohippo.com\/products\/primary-mouse-c57bi-6-hepatocytes-plateable-bhc10901835","provider":"BioHippo","version":"1.0","type":"link"}