{"product_id":"protein-and-dna-removal-spin-columns-bhu16300001","title":"Protein and DNA Removal Spin-Columns","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSpin Columns\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eProtein and DNA Removal Spin-Columns separate small molecules and liposomes from proteins, DNA and RNA. Proteins, nucleic acids and drugs bound to them stay on the column, while small polar molecules or liposomes pass into the eluate.\u003c\/p\u003e\n\u003cp\u003eTypical uses include removing DNA, RNA or proteins from biological samples before HPLC or other analysis, separating free and liposome-encapsulated drugs from protein-bound or DNA-bound drugs, and separating free ligands from receptor-bound ligands. Binding to the resin is mainly through charge-charge interactions; organic and inorganic phosphates may also bind to the column.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e20 columns – PNR020\u003c\/th\u003e\n\u003ctd\u003e20 pre-packed spin columns in 50% ethanol.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003ePre-packed spin columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Volume\u003c\/th\u003e\n\u003ctd\u003e0.2 ml per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding Capacity\u003c\/th\u003e\n\u003ctd\u003eMore than 100 µg of protein or 20 µg of DNA per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColumn Storage Solution\u003c\/th\u003e\n\u003ctd\u003e50% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEquipment Needed\u003c\/th\u003e\n\u003ctd\u003eBench-top microcentrifuge and 1.5 ml microcentrifuge tubes as receiver tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003ePNR020\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSpin each column briefly to settle the resin, remove the bottom tip and cap, and spin at 13,000 rpm for 1 min to remove the storage solution.\u003c\/li\u003e\n\u003cli\u003eWash with 200 µl of water (13,000 rpm, 1 min) and move the column to a clean 1.5 ml tube.\u003c\/li\u003e\n\u003cli\u003eLoad 200 µl of sample (in a buffer containing 100 mM NaCl), spin at 1,000 rpm for 1 min and then at 13,000 rpm for 1 min, and keep the eluate.\u003c\/li\u003e\n\u003cli\u003eSpin the eluate at 13,000 rpm for 1 min, or filter it through a 0.22 µm filter, to remove any insoluble material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Protein and DNA Removal Spin-columns” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIncluding 100 mM NaCl in the sample is not needed to remove protein or DNA, but it helps keep small charged molecules from binding to the column.\u003c\/li\u003e\n\u003cli\u003eLarger samples can be processed by repeating the loading step until the column binding capacity is reached.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"20 columns","offer_id":67815923450221,"sku":"PNR020","price":311.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/protein-and-dna-removal-spin-columns-bhu16300001","provider":"BioHippo","version":"1.0","type":"link"}