{"product_id":"protein-phosphatase-1-regulatory-subunit-15b-antibody-ppp1r15b-bha17129801","title":"Protein phosphatase 1 regulatory subunit 15B Antibody \/ PPP1R15B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eProtein phosphatase 1 regulatory subunit 15B Antibody \/ PPP1R15B is an antibody targeting \u003cstrong\u003ePPP1R15B\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PPP1R15B.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); Rabbit IgG.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB, FACS, Direct ELISA (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003ePPP1R15B (Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1 (PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of the PPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -\/- mice at a mendelian ratio. However, Ppp1r15b -\/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -\/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -\/- embryos with wildtype Eif2-alpha.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eFlow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry Q5SWA1 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q5SWA1 - Gene search: PPP1R15B — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPP1R15B - Ensembl search: PPP1R15B — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PPP1R15B - PubMed search: PPP1R15B antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPP1R15B+antibody - Reactome search: PPP1R15B — Reactome — https:\/\/reactome.org\/content\/query?q=PPP1R15B --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53046469329261,"sku":"RQ8244","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_58001a1b-7a34-47c3-9508-3f5283113dc6.jpg?v=1772000820","url":"https:\/\/www.ebiohippo.com\/products\/protein-phosphatase-1-regulatory-subunit-15b-antibody-ppp1r15b-bha17129801","provider":"BioHippo","version":"1.0","type":"link"}