{"product_id":"proteinase-k-bhz20800023","title":"Proteinase K","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eProteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg\/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eGenomic DNA extraction\u003c\/li\u003e\n\u003cli\u003eDigestion and removal of the enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"H3_backgro\"\u003eDerived from a genetically engineered yeast strain.\u003c\/li\u003e\n\u003cli class=\"H3_backgro\"\u003eFree of RNase and DNase contamination.\u003c\/li\u003e\n\u003cli class=\"H3_backgro\"\u003eMaintains stable activity in the presence of urea and SDS.\u003c\/li\u003e\n\u003cli class=\"H3_backgro\"\u003eActive across a broad pH range (pH 4.0 – 12.0), with optimal activity between pH 7.5 and 9.0.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eYeast\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecific Activity\u003c\/td\u003e\n\u003ctd\u003e≥30 U\/mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification\u003c\/td\u003e\n\u003ctd\u003eNi column affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction Condition\u003c\/td\u003e\n\u003ctd\u003eThe reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInactivation method\u003c\/td\u003e\n\u003ctd\u003eThe reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eActivity Range\u003c\/td\u003e\n\u003ctd\u003eIt has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase and DNase\u003c\/td\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.6665%;\"\u003eComponents No.\u003c\/td\u003e\n\u003ctd style=\"width: 20.8128%;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e10401ES60\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e10401ES80\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e10401ES90\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e10401ES98\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 21.6665%;\"\u003e10401\u003c\/td\u003e\n\u003ctd style=\"width: 20.8128%;\"\u003e\n\u003cdiv\u003e\n\u003cspan\u003eProteinase K Powder\u003c\/span\u003e\u003cspan\u003e（\u003c\/span\u003e\u003cspan\u003eRNase-Free, DNase-Free\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e100 mg\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e1 g\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e5 g\u003c\/td\u003e\n\u003ctd style=\"width: 14.3802%;\"\u003e100 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eThe product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 mg","offer_id":53294150975853,"sku":"10401ES60","price":95.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":53294155301229,"sku":"10401ES80","price":855.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10401.webp?v=1778112665","url":"https:\/\/www.ebiohippo.com\/products\/proteinase-k-bhz20800023","provider":"BioHippo","version":"1.0","type":"link"}