{"product_id":"prp4k-antibody-pre-mrna-processing-factor-4b-kinase-bha17135839","title":"PRP4K Antibody \/ Pre-mRNA-processing factor 4B kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRP4K Antibody \/ Pre-mRNA-processing factor 4B kinase is a anti-PRP4K Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRP4K\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRP4K antibody detects Pre-mRNA-processing factor 4B kinase, also known as PRP4 kinase (PRPF4B), a dual-specificity serine\/threonine-tyrosine kinase involved in pre-mRNA splicing and signal transduction. The UniProt recommended name is Serine\/threonine-protein kinase PRP4 homolog (PRPF4B), and alternate names include pre-mRNA-processing factor 4 homolog B, PRP4 kinase, and PRPF4-related kinase. PRP4K functions at the intersection of RNA processing and cell signaling, linking splicing machinery dynamics to stress and growth pathways.\u003cbr\u003e\u003cbr\u003ePRP4K antibody targets a nuclear kinase that associates with spliceosomal components and regulates phosphorylation of splicing factors such as SF3B1 and PRP6, influencing spliceosome assembly and mRNA maturation. PRP4K activity is essential for accurate exon recognition and alternative splicing decisions, affecting transcriptome diversity. The protein also modulates mitotic checkpoint signaling through interaction with BUB1 and MAD1 complexes, ensuring proper chromosome segregation. Dysregulation of PRPF4B expression has been associated with aberrant splicing events, genomic instability, and tumorigenesis.\u003cbr\u003e\u003cbr\u003eThe PRPF4B gene is located on chromosome 6p21.31 and encodes a protein of approximately 1007 amino acids containing an N-terminal kinase domain and a C-terminal proline-rich regulatory region. PRP4K phosphorylates SR-rich splicing proteins, coordinating RNA processing with transcriptional elongation and stress responses. Its activity is regulated by phosphorylation, autoinhibition, and interactions with 14-3-3 proteins. Elevated expression of PRP4K has been observed in cancers such as breast, ovarian, and glioblastoma, where it influences epithelial-mesenchymal transition (EMT) and drug resistance phenotypes.\u003cbr\u003e\u003cbr\u003eResearchers employ PRP4K antibody for western blotting, immunofluorescence, and immunoprecipitation to monitor nuclear localization, post-translational modifications, and spliceosomal associations. The kinase also participates in response to genotoxic stress and growth factor signaling via ERK and JNK cascades. Functional depletion studies reveal that loss of PRP4K impairs cell cycle progression and enhances sensitivity to DNA-damaging agents. Because PRP4K integrates splicing control with cell survival signaling, it has emerged as a potential therapeutic target for cancer intervention.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRP4K - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRP4K - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRP4K - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRP4K - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRP4K+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306355053,"sku":"FY12937","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_da40a0ad-c5aa-47fd-944f-9f3d1eec52e9.jpg?v=1782237058","url":"https:\/\/www.ebiohippo.com\/products\/prp4k-antibody-pre-mrna-processing-factor-4b-kinase-bha17135839","provider":"BioHippo","version":"1.0","type":"link"}