{"product_id":"prpf31-antibody-pre-mrna-processing-factor-31-bha17135942","title":"PRPF31 Antibody \/ Pre-mRNA processing factor 31","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRPF31 Antibody \/ Pre-mRNA processing factor 31 is a anti-PRPF31 Rabbit antibody Recombinant Rabbit Monoclonal clone 29P97 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRPF31\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29P97, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRPF31 antibody detects Pre-mRNA processing factor 31, encoded by the PRPF31 gene. Pre-mRNA processing factor 31 is a component of the spliceosome, the large ribonucleoprotein complex responsible for removing introns from precursor mRNAs. It is specifically part of the U4\/U6.U5 tri-snRNP complex, where it plays an essential role in spliceosome assembly and stability. PRPF31 antibody provides researchers with a critical reagent for studying splicing regulation, gene expression, and inherited retinal disease caused by PRPF31 mutations.\u003cbr\u003e\u003cbr\u003ePre-mRNA processing factor 31 is indispensable for the assembly of the U4\/U6.U5 tri-snRNP, which integrates into the spliceosome during pre-mRNA splicing. Research using PRPF31 antibody has demonstrated that loss of this protein disrupts tri-snRNP stability, preventing proper spliceosome activation and leading to defective intron removal. This highlights the importance of PRPF31 in maintaining gene expression fidelity and explains why its mutations cause tissue-specific disease despite its general role in splicing.\u003cbr\u003e\u003cbr\u003eMutations in PRPF31 are a well-established cause of autosomal dominant retinitis pigmentosa, a degenerative eye disease characterized by progressive photoreceptor cell death and vision loss. Studies with PRPF31 antibody have shown that reduced protein expression, often due to haploinsufficiency, leads to insufficient splicing activity in retinal cells. This impairs photoreceptor survival and contributes to disease progression. The link between PRPF31 and retinal degeneration underscores the importance of splicing factors in tissue-specific pathologies.\u003cbr\u003e\u003cbr\u003eBeyond retinitis pigmentosa, alterations in splicing machinery are increasingly recognized in cancer and neurodegeneration. PRPF31 antibody has been used to examine expression in tumors, where splicing factor mutations or dysregulation contribute to aberrant gene expression. In neurological disease, spliceosome defects are emerging as contributors to motor neuron degeneration and other pathologies. Studying Pre-mRNA processing factor 31 provides insight into how splicing fidelity influences cell fate and disease susceptibility.\u003cbr\u003e\u003cbr\u003ePRPF31 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms expression levels across tissues, immunohistochemistry highlights nuclear localization, and immunofluorescence shows colocalization with spliceosome markers. Functional studies using PRPF31 antibody include knockdown and rescue experiments to establish causal relationships between reduced PRPF31 and splicing defects. These diverse applications make it an indispensable tool for RNA biology research.\u003cbr\u003e\u003cbr\u003eBy providing validated PRPF31 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRPF31 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRPF31 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRPF31 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRPF31 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRPF31+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309435245,"sku":"FY13040","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b1c85ad9-2f94-46dc-8eb8-f3718aba6e15.jpg?v=1772019381","url":"https:\/\/www.ebiohippo.com\/products\/prpf31-antibody-pre-mrna-processing-factor-31-bha17135942","provider":"BioHippo","version":"1.0","type":"link"}