{"product_id":"prpf4-antibody-pre-mrna-processing-factor-4-bha17135257","title":"PRPF4 Antibody \/ Pre mRNA processing factor 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRPF4 Antibody \/ Pre mRNA processing factor 4 is a anti-PRPF4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P52 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRPF4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P52, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRPF4 antibody recognizes pre mRNA processing factor 4, a nuclear protein essential for spliceosome assembly and pre mRNA splicing. PRPF4 is a component of the U4 and U6 small nuclear ribonucleoproteins and is required for the formation of the U4 or U6 U5 tri small nuclear ribonucleoprotein complex. This complex is indispensable for pre mRNA splicing, a fundamental process that ensures accurate removal of introns and joining of exons to produce mature messenger RNA.\u003cbr\u003e\u003cbr\u003ePRPF4 antibody is highly relevant for studies of gene expression regulation and RNA biology. By detecting PRPF4, researchers can investigate how spliceosome components assemble and how splicing is regulated under different physiological conditions. Disruption of PRPF4 function impairs RNA processing and leads to widespread changes in gene expression, highlighting its importance in cell biology.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and other molecular biology applications. In western blot assays, PRPF4 antibody recognizes nuclear protein bands, confirming expression across tissue and cell samples. Immunohistochemistry provides tissue localization, often highlighting nuclear distribution in actively transcribing cells. Immunofluorescence further resolves subnuclear localization and colocalization with other spliceosomal proteins.\u003cbr\u003e\u003cbr\u003eMutations in PRPF4 are linked to retinitis pigmentosa, a degenerative retinal disorder characterized by progressive vision loss. This connection underscores the physiological importance of splicing factors beyond general RNA metabolism. PRPF4 antibody therefore provides a valuable reagent for studies of inherited retinal disease as well as broader investigations of splicing related disorders.\u003cbr\u003e\u003cbr\u003eSplicing dysregulation is also increasingly recognized in cancer and neurodegeneration. Aberrant splicing contributes to oncogenic transformation, resistance to therapy, and progression of neurodegenerative diseases. Detecting PRPF4 expression with specific antibodies enables researchers to connect splicing factor function with disease mechanisms and to evaluate therapeutic interventions targeting the spliceosome.\u003cbr\u003e\u003cbr\u003ePRPF4 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRPF4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRPF4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRPF4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRPF4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRPF4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047286890861,"sku":"FY12354","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6535406b-154d-4a01-b722-c517abdf9745.jpg?v=1772019283","url":"https:\/\/www.ebiohippo.com\/products\/prpf4-antibody-pre-mrna-processing-factor-4-bha17135257","provider":"BioHippo","version":"1.0","type":"link"}