{"product_id":"prpf4-antibody-pre-mrna-processing-factor-4-bha17135456","title":"PRPF4 Antibody \/ Pre mRNA processing factor 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePRPF4 Antibody \/ Pre mRNA processing factor 4 is a anti-PRPF4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P60 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PRPF4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P60, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePRPF4 antibody detects pre mRNA processing factor 4, a spliceosome associated protein encoded by the PRPF4 gene. PRPF4 is a component of the U4\/U6.U5 tri snRNP complex, which is required for pre mRNA splicing. By stabilizing interactions between snRNPs, PRPF4 contributes to spliceosome assembly and catalytic activation. This function makes PRPF4 essential for gene expression and RNA processing in all eukaryotic cells.\u003cbr\u003e\u003cbr\u003ePRPF4 antibody is widely used in molecular biology and genetic research. Mutations in PRPF4 are linked to retinitis pigmentosa, a degenerative eye disease characterized by progressive photoreceptor loss. By detecting PRPF4, researchers can study the molecular basis of splicing related diseases and investigate how splicing defects cause tissue specific pathology despite the ubiquitous nature of the spliceosome.\u003cbr\u003e\u003cbr\u003e In western blot assays, a PRPF4 antibody detects protein bands of the expected size in nuclear extracts. Immunohistochemistry reveals PRPF4 expression in retina and other tissues, while immunofluorescence highlights nuclear localization in splicing speckles. These applications support detailed study of spliceosomal proteins in cells and tissues.\u003cbr\u003e\u003cbr\u003ePRPF4 is critical for RNA splicing fidelity and gene expression regulation. Disruption of PRPF4 function alters transcriptome diversity and contributes to developmental abnormalities. Its role in retinitis pigmentosa underscores the importance of splicing factors in specialized tissues such as retina. By applying PRPF4 antibody, scientists can explore how mutations affect splicing and develop potential therapeutic strategies to restore RNA processing.\u003cbr\u003e\u003cbr\u003ePRPF4 also interacts with kinases and other splicing regulators, linking it to signaling pathways that modulate spliceosome dynamics. These interactions suggest broader roles for PRPF4 in cell cycle control and stress responses. As a result, PRPF4 antibody is valuable for investigating how splicing integrates with cellular physiology.\u003cbr\u003e\u003cbr\u003ePRPF4 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PRPF4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRPF4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRPF4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PRPF4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PRPF4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047292887405,"sku":"FY12553","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fab05fef-0155-4d85-9f5a-00a2158790b1.jpg?v=1772019305","url":"https:\/\/www.ebiohippo.com\/products\/prpf4-antibody-pre-mrna-processing-factor-4-bha17135456","provider":"BioHippo","version":"1.0","type":"link"}