{"product_id":"psip1-antibody-pc4-and-sfrs1-interacting-protein-ledgf-bha17135861","title":"PSIP1 Antibody \/ PC4 and SFRS1-interacting protein \/ LEDGF","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSIP1 Antibody \/ PC4 and SFRS1-interacting protein \/ LEDGF is a anti-PSIP1 Rabbit antibody Recombinant Rabbit Monoclonal clone 31P66 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSIP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31P66, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSIP1 antibody detects Lens epithelium derived growth factor, encoded by the PSIP1 gene. Lens epithelium derived growth factor, also known as LEDGF, is a transcription coactivator that plays important roles in stress response, survival signaling, and gene regulation. It binds chromatin through its PWWP domain and recruits transcriptional machinery to stress inducible genes, helping cells adapt to oxidative and environmental stress. PSIP1 antibody provides a means to study this protein's functions in transcriptional control, survival pathways, and viral integration.\u003cbr\u003e\u003cbr\u003eLens epithelium derived growth factor is best known for its role in HIV infection, where it acts as a tethering factor for integrase, directing viral DNA integration into active chromatin regions. Research using PSIP1 antibody has confirmed that depletion of LEDGF reduces viral replication efficiency, underscoring its importance in retroviral life cycles. Beyond virology, PSIP1 regulates stress response genes, including those activated by heat shock and oxidative stress, contributing to cellular resilience. By detecting this protein with PSIP1 antibody, researchers gain insight into how cells survive hostile environments.\u003cbr\u003e\u003cbr\u003ePSIP1 also influences development and cancer. Overexpression of LEDGF has been observed in leukemia and solid tumors, where it enhances survival pathways and confers resistance to apoptosis. Chromosomal translocations involving PSIP1 result in fusion proteins that act as oncogenic drivers, particularly in mixed lineage leukemia. Studies employing PSIP1 antibody have demonstrated altered transcriptional programs and stress resistance in tumor cells, linking LEDGF to oncogenesis and therapeutic resistance. Because of these functions, PSIP1 remains a focus in drug discovery and biomarker research.\u003cbr\u003e\u003cbr\u003ePSIP1 antibody is applied in western blotting, chromatin immunoprecipitation, and immunohistochemistry. Western blotting demonstrates isoform expression and stress inducible regulation. Chromatin immunoprecipitation with PSIP1 antibody identifies genomic targets of LEDGF, revealing pathways controlled by this coactivator. Immunohistochemistry highlights tissue distribution, while functional assays demonstrate its ability to modulate transcriptional activity under stress. These diverse applications underscore the importance of PSIP1 antibody in molecular and cellular research.\u003cbr\u003e\u003cbr\u003eBy supplying validated PSIP1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSIP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSIP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSIP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSIP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSIP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047307174253,"sku":"FY12959","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_03bcc828-4bd2-4a25-8636-4d8e717d4e78.jpg?v=1772019369","url":"https:\/\/www.ebiohippo.com\/products\/psip1-antibody-pc4-and-sfrs1-interacting-protein-ledgf-bha17135861","provider":"BioHippo","version":"1.0","type":"link"}