{"product_id":"psma5-antibody-proteasome-subunit-alpha-type-5-bha17136358","title":"PSMA5 Antibody \/ Proteasome subunit alpha type 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMA5 Antibody \/ Proteasome subunit alpha type 5 is a anti-PSMA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat, Monkey. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMA5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMA5 antibody is a valuable reagent for studying protein degradation and cellular homeostasis. The encoded protein, proteasome subunit alpha type 5, is a core component of the 20S proteasome, a large multi-catalytic complex responsible for degrading ubiquitinated proteins. The proteasome plays a central role in maintaining protein quality control, regulating cell cycle progression, and modulating signaling pathways. PSMA5 belongs to the alpha subunit family, which forms part of the structural gate controlling substrate entry into the catalytic chamber of the proteasome.\u003cbr\u003e\u003cbr\u003ePSMA5 contributes to the assembly and stability of the proteasome complex. The alpha subunits, including PSMA5, form a heptameric ring that shapes one end of the barrel-like core. This arrangement functions as a regulated gate, opening to allow access of protein substrates targeted for degradation. By influencing the selectivity and efficiency of protein turnover, PSMA5 is critical for cellular responses to stress, metabolic regulation, and removal of damaged or misfolded proteins.\u003cbr\u003e\u003cbr\u003eResearch into PSMA5 has highlighted its importance in diverse biological processes. Proteasome activity, dependent on components such as PSMA5, is required for antigen processing, generating peptides that are presented by MHC class I molecules to cytotoxic T lymphocytes. This immune surveillance mechanism is essential for identifying and destroying infected or transformed cells. In addition, proteasome function regulates transcription factors, signaling proteins, and cell cycle regulators, placing PSMA5 at the heart of pathways that determine proliferation, differentiation, and apoptosis.\u003cbr\u003e\u003cbr\u003eAlterations in proteasome activity have been associated with cancer, neurodegenerative disorders, and inflammatory disease. Dysregulation of PSMA5 expression or proteasome activity can disrupt protein turnover, contributing to abnormal signaling and accumulation of toxic protein aggregates. Pharmacologic inhibitors of the proteasome, such as bortezomib, underscore the therapeutic relevance of targeting this pathway, with PSMA5 as a structural and functional element within the proteasome complex.\u003cbr\u003e\u003cbr\u003eThe PSMA5 antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels and subcellular localization of this proteasome subunit. Such applications are essential for assessing changes in protein degradation capacity across tissues, during disease progression, or under experimental manipulation. For scientists investigating protein quality control, immune responses, or therapeutic intervention strategies, the PSMA5 antibody provides a reliable and precise detection tool.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMA5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMA5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMA5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMA5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMA5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047325884781,"sku":"RQ8940","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c8428306-4e9b-4be5-9e1b-32ecb71e298e.jpg?v=1772019450","url":"https:\/\/www.ebiohippo.com\/products\/psma5-antibody-proteasome-subunit-alpha-type-5-bha17136358","provider":"BioHippo","version":"1.0","type":"link"}