{"product_id":"psmb10-antibody-proteasome-subunit-beta-type-10-bha17136134","title":"PSMB10 Antibody \/ Proteasome subunit beta type 10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMB10 Antibody \/ Proteasome subunit beta type 10 is a anti-PSMB10 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P25 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMB10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P25, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMB10 antibody detects Proteasome subunit beta type-10, encoded by the PSMB10 gene. Proteasome subunit beta type-10 is a catalytic component of the immunoproteasome, which processes intracellular proteins for presentation by MHC class I molecules. PSMB10 antibody provides researchers with a highly specific reagent to study antigen processing, immune responses, and proteasome biology.\u003cbr\u003e\u003cbr\u003eThe proteasome is a multicatalytic protease complex responsible for degrading ubiquitinated proteins. Research using PSMB10 antibody has demonstrated that incorporation of Proteasome subunit beta type-10 into the 20S proteasome replaces the standard catalytic subunit beta type-2, conferring altered cleavage specificity optimized for generating peptides that bind MHC class I molecules. This substitution enhances immune surveillance by producing peptides with anchor residues suited for antigen presentation.\u003cbr\u003e\u003cbr\u003ePSMB10 is inducible by interferon gamma, which promotes immunoproteasome assembly in response to infection. Studies with PSMB10 antibody have shown that upregulation of this subunit increases antigenic peptide supply, facilitating recognition of infected or malignant cells by cytotoxic T lymphocytes. This mechanism highlights the link between interferon signaling, proteasome remodeling, and adaptive immunity.\u003cbr\u003e\u003cbr\u003eBeyond its role in immunity, altered expression of Proteasome subunit beta type-10 has been implicated in disease. Research using PSMB10 antibody has revealed that dysregulation of immunoproteasome activity contributes to autoimmunity, cancer, and neurodegeneration. Overactive immunoproteasome function can enhance self-antigen presentation, driving autoimmunity, while impaired activity reduces antigen presentation and immune defense. These findings make PSMB10 a critical focus in both basic and translational research.\u003cbr\u003e\u003cbr\u003ePSMB10 antibody is applied in western blotting, immunohistochemistry, and flow cytometry. Western blotting confirms immunoproteasome incorporation, immunohistochemistry highlights tissue-specific expression, and flow cytometry quantifies levels in immune cells. These applications make PSMB10 antibody essential in immunology and proteostasis research.\u003cbr\u003e\u003cbr\u003eBy supplying validated PSMB10 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMB10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMB10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMB10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMB10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMB10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047319265645,"sku":"FY13232","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c199ccc0-84d2-44be-8d46-84efe26cebfc.jpg?v=1772019412","url":"https:\/\/www.ebiohippo.com\/products\/psmb10-antibody-proteasome-subunit-beta-type-10-bha17136134","provider":"BioHippo","version":"1.0","type":"link"}