{"product_id":"psmc1-antibody-26s-proteasome-regulatory-subunit-4-bha17136359","title":"PSMC1 Antibody \/ 26S Proteasome regulatory subunit 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMC1 Antibody \/ 26S Proteasome regulatory subunit 4 is a anti-PSMC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMC1 antibody is an important research tool for studying protein degradation, signaling, and cellular homeostasis. The encoded protein, 26S proteasome regulatory subunit 4, is a member of the AAA ATPase family and serves as a key component of the 19S regulatory particle that associates with the 20S core to form the 26S proteasome. This large multi-subunit protease complex is central to ubiquitin-dependent protein degradation, ensuring timely turnover of regulatory proteins and removal of damaged or misfolded proteins.\u003cbr\u003e\u003cbr\u003ePSMC1, also known as Rpt2, is one of six ATPases that form a heterohexameric ring in the base of the 19S regulatory particle. Through ATP hydrolysis, PSMC1 contributes to unfolding ubiquitinated substrates and translocating them into the 20S catalytic chamber for degradation. This activity is essential for controlling protein quality, regulating the cell cycle, modulating transcription factor activity, and coordinating stress responses. By ensuring efficient substrate processing, PSMC1 plays a fundamental role in preserving cellular function under both normal and stress conditions.\u003cbr\u003e\u003cbr\u003eThe proteasome system, which depends on subunits such as PSMC1, regulates diverse pathways including antigen processing for MHC class I presentation. This immune-related function is critical for defense against pathogens and recognition of abnormal cells. In addition, proteasome-mediated degradation influences signaling cascades involving NF-kB, p53, and cyclins, linking PSMC1 directly to processes such as apoptosis, proliferation, and DNA repair.\u003cbr\u003e\u003cbr\u003eAltered proteasome activity has been associated with cancer, neurodegenerative disorders, and autoimmune disease. Changes in expression or activity of PSMC1 may disrupt protein turnover, contributing to accumulation of toxic protein aggregates or dysregulated signaling. As proteasome inhibitors have already proven valuable in cancer therapy, individual components like PSMC1 are of increasing interest for their roles in disease biology and therapeutic targeting.\u003cbr\u003e\u003cbr\u003eThe PSMC1 antibody is widely applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to study protein expression, localization, and regulation. These applications allow researchers to investigate proteasome function in health and disease, assess subunit composition, and explore the effects of genetic or pharmacologic manipulation. For investigators examining protein quality control, immune surveillance, or therapeutic mechanisms, the PSMC1 antibody offers a reliable detection tool.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047327195501,"sku":"RQ8941","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4a617964-b3ae-4bbe-a290-bee3e5537317.jpg?v=1772019449","url":"https:\/\/www.ebiohippo.com\/products\/psmc1-antibody-26s-proteasome-regulatory-subunit-4-bha17136359","provider":"BioHippo","version":"1.0","type":"link"}