{"product_id":"psmc3-antibody-proteasome-26s-subunit-atpase-3-tbp-1-bha17136513","title":"Psmc3 Antibody \/ Proteasome 26S subunit ATPase 3 \/ Tbp-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePsmc3 Antibody \/ Proteasome 26S subunit ATPase 3 \/ Tbp-1 is a anti-PSMC3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eZebrafish Psmc3, also known as proteasome 26S subunit ATPase 3, is a critical component of the 19S regulatory particle of the 26S proteasome. Psmc3 is an ATP binding protein that participates in the recognition, unfolding, and translocation of ubiquitinated substrates into the proteolytic core for degradation. This function is essential for maintaining protein homeostasis, regulating cell cycle progression, and controlling key signaling pathways through targeted protein turnover.\u003cp\u003eZebrafish Psmc3 is an ortholog of the human PSMC3 protein, sharing high sequence similarity and functional conservation. In both zebrafish and humans, Psmc3 contributes to the ATP dependent steps required for proteasome assembly and substrate processing. This evolutionary conservation makes zebrafish a suitable model for studying the roles of Psmc3 in development, stress responses, and diseases linked to impaired proteasome function, such as neurodegeneration and cancer.\u003c\/p\u003e\n\u003cp\u003eThe use of a Zebrafish Psmc3 antibody allows researchers to detect and study the expression of Psmc3 in various tissues and developmental stages. These antibodies are valuable tools for applications including western blot, immunohistochemistry, and immunofluorescence, helping to clarify how proteasome activity is regulated in zebrafish. A Zebrafish Psmc3 antibody is also useful for comparative studies on proteasome regulation between zebrafish and mammalian systems.\u003c\/p\u003e\n\u003cp\u003eAt this time there are no known isoforms of zebrafish Psmc3. By using a Zebrafish Psmc3 antibody, researchers can investigate its contribution to protein quality control and understand its functional relevance to human health and disease.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047331946861,"sku":"RZ1319","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5f8a7bc8-ca1b-400e-9a76-a1a8761154eb.jpg?v=1772019476","url":"https:\/\/www.ebiohippo.com\/products\/psmc3-antibody-proteasome-26s-subunit-atpase-3-tbp-1-bha17136513","provider":"BioHippo","version":"1.0","type":"link"}