{"product_id":"psmc4-antibody-26s-proteasome-regulatory-subunit-6b-tbp-7-bha17135768","title":"PSMC4 Antibody \/ 26S proteasome regulatory subunit 6B \/ TBP-7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMC4 Antibody \/ 26S proteasome regulatory subunit 6B \/ TBP-7 is a anti-PSMC4 Rabbit antibody Recombinant Rabbit Monoclonal clone 31P63 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31P63, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMC4 antibody recognizes the 26S proteasome regulatory subunit 6B, also known as TBP-7, a protein encoded by the PSMC4 gene. This subunit is a member of the AAA ATPase family and forms part of the 19S regulatory particle of the 26S proteasome. The proteasome is the major protein degradation machinery in eukaryotic cells, responsible for removing damaged, misfolded, or short-lived regulatory proteins through ubiquitin-mediated proteolysis. PSMC4 antibody provides researchers with a key tool to study proteasomal structure and function, particularly in pathways linked to protein turnover, cell cycle regulation, and stress responses.\u003cbr\u003e\u003cbr\u003eThe 26S proteasome consists of a 20S catalytic core and a 19S regulatory complex. PSMC4 is one of six ATPase subunits within the base of the 19S regulator. These ATPases act as a molecular motor, unfolding ubiquitinated substrates and translocating them into the catalytic chamber for degradation. The role of PSMC4 is critical for energy-dependent substrate processing. Detection with PSMC4 antibody supports studies on proteasome assembly, activity, and substrate specificity.\u003cbr\u003e\u003cbr\u003eDysfunction of proteasome regulation has been linked to neurodegenerative diseases, cancer, and autoimmune disorders. Mutations or altered expression of proteasome subunits, including PSMC4, can disrupt proteostasis and lead to the accumulation of toxic protein aggregates. PSMC4 antibody has therefore been used to examine proteasome activity in conditions such as Parkinson disease, Huntington disease, and multiple myeloma. In cancer biology, changes in proteasome function influence cell proliferation and apoptosis, making it a therapeutic target.\u003cbr\u003e\u003cbr\u003ePSMC4 antibody is suitable for use in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting reveals subunit expression within the proteasome complex, while immunohistochemistry highlights localization in tissues with high proteolytic activity, such as liver and brain. Immunoprecipitation using PSMC4 antibody aids in proteasome purification and analysis of protein-protein interactions.\u003cbr\u003e\u003cbr\u003eResearchers studying pathways of ubiquitin-mediated degradation, antigen presentation, and stress responses rely on antibodies such as PSMC4 antibody to track proteasome composition and function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047305109869,"sku":"FY12866","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_20323d97-5c6e-4be6-8046-15b0543b8736.jpg?v=1772019357","url":"https:\/\/www.ebiohippo.com\/products\/psmc4-antibody-26s-proteasome-regulatory-subunit-6b-tbp-7-bha17135768","provider":"BioHippo","version":"1.0","type":"link"}