{"product_id":"psmc5-antibody-26s-proteasome-regulatory-subunit-8-bha17135923","title":"PSMC5 Antibody \/ 26S proteasome regulatory subunit 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMC5 Antibody \/ 26S proteasome regulatory subunit 8 is a anti-PSMC5 Rabbit antibody Recombinant Rabbit Monoclonal clone 29P84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMC5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29P84, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMC5 antibody detects 26S proteasome regulatory subunit 8, encoded by the PSMC5 gene. This protein is one of the six ATPases of the AAA family that form the base of the 19S regulatory particle of the proteasome. PSMC5, also known as TBP1, provides ATP hydrolysis required for unfolding and translocating ubiquitinated substrates into the 20S core particle for degradation. PSMC5 antibody provides researchers with a reliable tool to study protein homeostasis, cell cycle regulation, and signaling pathways dependent on proteasomal degradation.\u003cbr\u003e\u003cbr\u003eThe 26S proteasome is responsible for the majority of regulated intracellular protein degradation in eukaryotic cells. By removing misfolded proteins, damaged proteins, and short lived regulatory proteins, the proteasome maintains proteostasis and controls key signaling events. Research using PSMC5 antibody has shown that 26S proteasome regulatory subunit 8 contributes to ATP driven unfolding, ensuring substrate entry into the proteolytic chamber. This function is essential for turnover of cell cycle regulators, transcription factors, and signal transduction intermediates.\u003cbr\u003e\u003cbr\u003eDysfunction of the proteasome contributes to numerous diseases, including neurodegenerative disorders such as Parkinson and Alzheimer disease, where accumulation of protein aggregates is a hallmark. Research with PSMC5 antibody has demonstrated that altered proteasome activity leads to impaired clearance of aggregation prone proteins. In cancer, hyperactivation of the proteasome allows degradation of tumor suppressors and cell cycle inhibitors, promoting unchecked proliferation. PSMC5 has also been implicated in immune regulation, where proteasomal processing generates antigenic peptides presented on MHC class I molecules.\u003cbr\u003e\u003cbr\u003ePSMC5 antibody is widely used in western blotting, immunoprecipitation, and immunohistochemistry. Western blotting demonstrates abundance in metabolically active tissues, while immunoprecipitation allows isolation of 19S regulatory complexes containing PSMC5. Immunohistochemistry highlights proteasome distribution in brain, muscle, and immune organs. These applications make PSMC5 antibody versatile in both fundamental and translational research.\u003cbr\u003e\u003cbr\u003eBy supplying validated PSMC5 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMC5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMC5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMC5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMC5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMC5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309893997,"sku":"FY13021","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6649122e-af7c-41ac-860f-ba9f267d9af1.jpg?v=1772019379","url":"https:\/\/www.ebiohippo.com\/products\/psmc5-antibody-26s-proteasome-regulatory-subunit-8-bha17135923","provider":"BioHippo","version":"1.0","type":"link"}