{"product_id":"psmd10-antibody-gankyrin-bha17135260","title":"PSMD10 Antibody \/ Gankyrin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMD10 Antibody \/ Gankyrin is a anti-PSMD10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PSMD10 antibody targets 26S proteasome non-ATPase regulatory subunit 10, also known as Gankyrin. Encoded by the PSMD10 gene, this oncoprotein functions as a regulatory component of the 19S proteasome and as an adaptor in cell cycle and tumor signaling. 26S proteasome non-ATPase regulatory subunit 10 promotes degradation of key regulatory proteins, including p53 and retinoblastoma protein (Rb), thereby influencing cell proliferation, differentiation, and oncogenic transformation. The PSMD10 antibody provides a powerful reagent for investigating proteasome dynamics, tumorigenesis, and protein degradation mechanisms.\u003cbr\u003e\u003cbr\u003eGankyrin contains multiple ankyrin repeats that mediate protein–protein interactions with proteasome subunits and cell cycle regulators. It associates with CDK4, MDM2, and the 26S proteasome base complex to coordinate protein turnover. By enhancing MDM2-mediated ubiquitination of p53, 26S proteasome non-ATPase regulatory subunit 10 reduces tumor suppressor activity and contributes to cellular transformation. The PSMD10 antibody enables direct detection of Gankyrin expression in tumor tissues and cultured cells, supporting studies of its oncogenic potential and correlation with clinical outcomes.\u003cbr\u003e\u003cbr\u003eOverexpression of Gankyrin has been observed in numerous cancers, including hepatocellular carcinoma, colorectal carcinoma, and breast cancer. It accelerates cell cycle progression, promotes resistance to apoptosis, and enhances metastatic potential. The PSMD10 antibody allows researchers to quantify expression levels and subcellular localization of this protein, helping elucidate its functional role in oncogenic signaling pathways. Downregulation or inhibition of 26S proteasome non-ATPase regulatory subunit 10 has been shown to restore p53 stability and suppress tumor growth, making it an appealing therapeutic target.\u003cbr\u003e\u003cbr\u003eBeyond its oncogenic properties, Gankyrin contributes to proteasome assembly and quality control. It stabilizes interactions between ATPase and non-ATPase subunits of the 19S regulatory particle, facilitating efficient protein degradation. The PSMD10 antibody is suitable for western blotting, immunoprecipitation, and immunohistochemistry, allowing characterization of proteasomal function in normal and malignant cells. Its application extends to studies of proteostasis, cell cycle control, and ubiquitin-mediated degradation pathways.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286366573,"sku":"FY12357","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0e462fb1-b7be-4b56-b804-83b8c94c2440.jpg?v=1782236997","url":"https:\/\/www.ebiohippo.com\/products\/psmd10-antibody-gankyrin-bha17135260","provider":"BioHippo","version":"1.0","type":"link"}