{"product_id":"psmd5-antibody-26s-proteasome-non-atpase-regulatory-subunit-5-bha17136101","title":"PSMD5 Antibody \/ 26S proteasome non-ATPase regulatory subunit 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSMD5 Antibody \/ 26S proteasome non-ATPase regulatory subunit 5 is a anti-PSMD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSMD5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSMD5 antibody detects 26S proteasome non-ATPase regulatory subunit 5, a proteasome assembly modulator that regulates protein degradation and cellular proteostasis. The UniProt recommended name is 26S proteasome non-ATPase regulatory subunit 5 (PSMD5). Also known as Proteasome inhibitor PI31 homolog, this protein acts as a negative regulator of 26S proteasome formation and function.\u003cbr\u003e\u003cbr\u003eFunctionally, PSMD5 antibody identifies a 328-amino-acid cytoplasmic protein that binds proteasome regulatory subunits and interferes with their association into the mature 26S proteasome. PSMD5 fine-tunes proteasome activity by limiting the assembly of regulatory particles with the catalytic core, maintaining balance between protein degradation and stability. This regulation is crucial for cell cycle control, stress response, and antigen presentation.\u003cbr\u003e\u003cbr\u003eThe PSMD5 gene is located on chromosome 9q33.3 and is expressed in many tissues, including heart, brain, and skeletal muscle. PSMD5 expression is induced by stress and inflammatory signals to transiently reduce proteasome activity, preventing excessive protein turnover during cellular adaptation.\u003cbr\u003e\u003cbr\u003ePathologically, PSMD5 dysfunction affects protein quality control and contributes to neurodegenerative diseases, inflammation, and cancer. Overexpression inhibits proteasome activity, leading to accumulation of misfolded proteins, while deficiency may accelerate degradation of key signaling molecules. Research using PSMD5 antibody supports studies in proteostasis, protein degradation, and stress response pathways.\u003cbr\u003e\u003cbr\u003ePSMD5 antibody is validated for western blotting, immunofluorescence, and immunoprecipitation to detect proteasome regulatory subunits. \u003cbr\u003e\u003cbr\u003eStructurally, 26S proteasome non-ATPase regulatory subunit 5 contains a conserved PI31 domain responsible for proteasome binding and inhibitory function. This domain interacts with the base subcomplex of the regulatory particle, competing with normal assembly factors. This antibody enables detailed study of PSMD5's role in proteasome regulation and protein degradation dynamics.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSMD5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSMD5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSMD5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSMD5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSMD5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047317823853,"sku":"FY13199","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4a8dd6be-2ecf-4bf8-baa4-999499094e7f.jpg?v=1782237104","url":"https:\/\/www.ebiohippo.com\/products\/psmd5-antibody-26s-proteasome-non-atpase-regulatory-subunit-5-bha17136101","provider":"BioHippo","version":"1.0","type":"link"}