{"product_id":"psme3-antibody-proteasome-activator-complex-subunit-3-bha17135672","title":"PSME3 Antibody \/ Proteasome activator complex subunit 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSME3 Antibody \/ Proteasome activator complex subunit 3 is a anti-PSME3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, possible cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSME3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSME3 antibody detects Proteasome activator complex subunit 3 (also known as PA28 gamma or REG gamma), a nuclear proteasome regulator involved in ubiquitin-independent protein degradation, cell cycle control, and stress responses. Encoded by the PSME3 gene on chromosome 17q21.2, this protein belongs to the 11S (PA28) family of proteasome activators that modulate the 20S core particle. Unlike its cytoplasmic counterparts PA28 alpha and beta, which form the PA28 alpha\/beta complex activated by interferon-gamma, PSME3 functions mainly within the nucleus, where it regulates the turnover of transcriptional regulators, cyclins, and damaged proteins in a ubiquitin-independent manner.\u003cbr\u003e\u003cbr\u003ePSME3 forms homoheptameric rings that cap the 20S proteasome, enhancing peptide entry and release. This activation facilitates the degradation of short-lived or misfolded proteins without requiring ubiquitination or ATP. The protein is dynamically regulated through phosphorylation and interaction with other nuclear components, enabling rapid responses to cell stress or metabolic shifts. Key substrates of PSME3 include p21, p53, and steroid receptor coactivators, indicating its dual role in proteostasis and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eThe PSME3 antibody is widely used in cell biology, oncology, and proteasome regulation research to assess nuclear proteasome activity and protein turnover. Western blot analysis identifies a 29 kilodalton band corresponding to PA28 gamma, and immunofluorescence reveals diffuse nuclear staining. This antibody supports studies of proteolytic regulation in proliferation, apoptosis, and DNA damage responses. Because PSME3 controls the degradation of regulatory proteins involved in the cell cycle and tumor suppression, its dysfunction is associated with oncogenesis and altered stress tolerance.\u003cbr\u003e\u003cbr\u003eIn cancer, overexpression of PSME3 promotes tumor progression by accelerating degradation of p53 and p21, thereby weakening cell-cycle checkpoints and apoptosis. Conversely, loss of PSME3 function leads to accumulation of damaged proteins and proteostasis stress. Beyond cancer, PSME3 contributes to chromatin regulation, immune surveillance, and viral infection control. The PSME3 antibody enables researchers to examine these diverse processes by providing reliable detection in western blot, immunofluorescence, and immunohistochemistry.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSME3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSME3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSME3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSME3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSME3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047300161901,"sku":"FY12769","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7f20d166-8deb-43c8-9697-d44b8eb4b1f3.jpg?v=1782237038","url":"https:\/\/www.ebiohippo.com\/products\/psme3-antibody-proteasome-activator-complex-subunit-3-bha17135672","provider":"BioHippo","version":"1.0","type":"link"}