{"product_id":"pstpip1-antibody-pest-phosphatase-interacting-protein-1-bha17135390","title":"PSTPIP1 Antibody \/ PEST phosphatase-interacting protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePSTPIP1 Antibody \/ PEST phosphatase-interacting protein 1 is a anti-PSTPIP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PSTPIP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePSTPIP1 antibody detects Proline-serine-threonine phosphatase-interacting protein 1, a cytoskeletal adaptor protein that regulates actin dynamics, immune signaling, and inflammasome activation. PSTPIP1 is primarily expressed in hematopoietic cells, where it interacts with the actin cytoskeleton and tyrosine phosphatases to coordinate immune cell morphology and migration. The PSTPIP1 antibody is extensively used in immunology and inflammation research to study cytoskeletal remodeling and autoinflammatory disease mechanisms.\u003cbr\u003e\u003cbr\u003ePSTPIP1 is encoded by the PSTPIP1 gene located on human chromosome 15q24.3. The protein belongs to the F-BAR domain-containing family and features an N-terminal F-BAR domain that mediates membrane curvature and actin binding, along with a C-terminal SH3 domain that interacts with proline-rich regions in partner proteins such as PEST-type tyrosine phosphatases and pyrin. Through these interactions, PSTPIP1 regulates membrane deformation, phagocytosis, and signaling cascades in macrophages and neutrophils.\u003cbr\u003e\u003cbr\u003eThe PSTPIP1 antibody typically detects a 48-52 kilodalton protein by western blot and produces strong cytoplasmic and membrane-associated staining in immune cells. Functionally, PSTPIP1 links the actin cytoskeleton to signaling complexes, modulating processes such as cell spreading, migration, and inflammasome assembly. Mutations in PSTPIP1 are associated with PAPA (pyogenic arthritis, pyoderma gangrenosum, and acne) syndrome and other autoinflammatory disorders, where altered protein-protein interactions lead to hyperactivation of the pyrin inflammasome and excessive interleukin-1 beta production.\u003cbr\u003e\u003cbr\u003ePSTPIP1 interacts with proteins including WASP, c-Abl kinase, and SHIP1, integrating actin dynamics with immune receptor signaling. In macrophages, it contributes to the formation of podosomes and phagocytic structures. Beyond immune regulation, PSTPIP1 participates in bone remodeling and osteoclast differentiation through cytoskeletal control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PSTPIP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PSTPIP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PSTPIP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PSTPIP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PSTPIP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289676141,"sku":"FY12487","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7f1ebea0-fde6-42c7-901c-1e19c0fca6d2.jpg?v=1782237004","url":"https:\/\/www.ebiohippo.com\/products\/pstpip1-antibody-pest-phosphatase-interacting-protein-1-bha17135390","provider":"BioHippo","version":"1.0","type":"link"}